LOCUS       sequence-164791-         985 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E.coli cloning and expression vector. Has cloning sites downstream 
            of Tet promoter. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 985)
  TITLE     Coleman Lab plasmids
REFERENCE   2  (bases 1 to 985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pUS212
FEATURES             Location/Qualifiers
     source          1..985
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     223..240
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(264..321)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    322..342
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     promoter        358..413
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    359..377
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    389..407
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     primer_bind     complement(505..524)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
       61 ctgtggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
      121 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
      181 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
      241 gaagcctgca taacgcgaag taatcttttc ggttttaaag aaaaagggca gggtggtgac
      301 accttgccct tttttgccgg actgcagcgg ccgctactag tactattaaa ttcacttttc
      361 tctatcactg atagggagtg gtaaaataac tctatcaatg atagagtgtc aacaaaaatt
      421 aggaattaat gatgtccaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc
      481 ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag
      541 agcagcctac attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca
      601 ttgagatgtt agataggcac catactcact tttgcccttt agaaggggaa agctggcaag
      661 attttttacg taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag
      721 caaaagtaca tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat
      781 tagccttttt atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg
      841 tggggcattt tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag
      901 aaagggaaac acctactact gatagtatgc cgccattatt acgacaagct atcgaattat
      961 ttgatcacca aggtgcagag ccagc
//
