LOCUS       sequence-164793-         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E.coli cloning and expression vector. Has cloning sites downstream 
            of Tet promoter. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  TITLE     Coleman Lab plasmids
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pUS212
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     217..236
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     464..482
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(486..529)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    532..553
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc
       61 tcaccgtctt tcattgccat acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg
      121 tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta
      181 atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa
      241 tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc
      301 attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat
      361 cttatttcat tatggtgaaa gttggaacct cttacgtgcc cgatcaactc gagtgccacc
      421 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
      481 gcagaatttc agataaaaaa aatccttagc tttcgctaag gatgatttct ggaattcgcg
      541 gccgcttcta gagatcacgg aaaaaggtta tgctgctttt aagacccact ttcacattta
      601 agttgttttt ctaatccgca tatgatcaat tcaaggccga ataagaaggc tggctctgca
      661 ccttggtgat caaataattc gatagcttgt cgtaataatg gcggcatact atcagtagta
      721 ggtgtttccc tttcttcttt agcgacttga tgctcttgat cttccaatac gcaacctaaa
      781 gtaaaatgcc ccacagcgct gagtgcatat aatgcattct ctagtgaaaa accttgttgg
      841 cataaaaagg ctaattgatt ttcgagagtt tcatactgtt tttctgtagg ccgtgtacct
      901 aaatgtactt ttgctccatc gcgatgactt agtaaagcac atctaaaact
//
