LOCUS       sequence-164796-        1017 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GST-CD (Cbx7) fusion proteins in E. coli. Cbx7 
            chromodomain only; amino acids 1–62..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1017)
  TITLE     pTRIPZ-MSC1-Halotag-Cbx2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pGEX-6P-1-GST-CD(Cbx7)
FEATURES             Location/Qualifiers
     source          1..1017
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     121..143
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(417..440)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(467..489)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg
       61 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
      121 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagatcg
      181 tcagtcagtc acgatgcggc cgctcgagct cactcctcct tctcctcgta ggccatgacg
      241 aggcgggggt ccaagatgtg ctcttctggc tcccacgtgc tgtactttgg gggccatcct
      301 ttccacttca ccagatactc gactttaccc ttccgcacgc gcttcttccg gatgctctcc
      361 acggcgaaca cctgctcgcc gatggctgac agctccatga attccgggga tcccaggggc
      421 ccctggaaca gaacttccag atccgatttt ggaggatggt cgccaccacc aaacgtggct
      481 tgccagccct gcaaaggcca tgctatatac ttgctggatt tcaagtactt atcaatttgt
      541 gggatagctt caatacgttt tttaaaacaa actaattttg ggaacgcatc caggcacatt
      601 gggtccatgt ataaaacaac atcaagagcg tcatacaaca tgaagtcagg atgggttaca
      661 tgatcaccat ttaaatatgt tttatgacat aaacgatctt cgaacatttt cagcatttca
      721 ggtagcttgc taagaaaatc aactttgaga gtttcaaagt ctttactata tgcaattctc
      781 gaaacaccgt atctaatatc caaaaccgct ccttcaagca ttgaaatctc tgcacgctct
      841 tttggacaac cacccaacat gttgtgcttg tcagctatat aacgtatgat ggccatagac
      901 tgtgttaatt taacatcacc atcaatataa taaggaagat tgggaaactc caaacccaat
      961 tcaaactttt tgtttcgcca tttatcacct tcatcgcgct catacaaatg ctcttca
//
