LOCUS       sequence-164798-        1044 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GST-CD (Cbx7) fusion proteins in E. coli. Cbx7 ATL region 
            only, amino acids 66–81..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1044)
  TITLE     pTRIPZ-MSC1-Halotag-Cbx2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pGEX-6P-1-GST-ATL(Cbx7)
FEATURES             Location/Qualifiers
     source          1..1044
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             10..33
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(169..191)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(336..354)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        422..526
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(745..764)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aaatcggatc tggaagttct gttccagggg cccctgggat ccccggaatt cgcatcgggg
       61 tataggaaga gaggtccgaa acccaagcgg cttctgctgt gagctcgagc ggccgcatcg
      121 tgactgactg acgatctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
      181 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
      241 tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag
      301 cgatagcgga gtgtataatt cttgaagacg aaagggcctc gtgatacgcc tatttttata
      361 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
      421 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
      481 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
      541 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
      601 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
      661 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
      721 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg
      781 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
      841 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
      901 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
      961 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     1021 ggagctgaat gaagccatac caaa
//
