LOCUS       sequence-164799-         813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GST-CD (Cbx7) fusion proteins in E. coli. Cbx7 ATL region 
            only, amino acids 66–81..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  TITLE     pTRIPZ-MSC1-Halotag-Cbx2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pGEX-6P-1-GST-ATL(Cbx7)
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     121..143
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(279..302)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(329..351)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg
       61 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
      121 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagatcg
      181 tcagtcagtc acgatgcggc cgctcgagct cacagcagaa gccgcttggg tttcggacct
      241 ctcttcctat accccgatgc gaattccggg gatcccaggg gcccctggaa cagaacttcc
      301 agatccgatt ttggaggatg gtcgccacca ccaaacgtgg cttgccagcc ctgcaaaggc
      361 catgctatat acttgctgga tttcaagtac ttatcaattt gtgggatagc ttcaatacgt
      421 tttttaaaac aaactaattt tgggaacgca tccaggcaca ttgggtccat gtataaaaca
      481 acatcaagag cgtcatacaa catgaagtca ggatgggtta catgatcacc atttaaatat
      541 gttttatgac ataaacgatc ttcgaacatt ttcagcattt caggtagctt gctaagaaaa
      601 tcaactttga gagtttcaaa gtctttacta tatgcaattc tcgaaacacc gtatctaata
      661 tccaaaaccg ctccttcaag cattgaaatc tctgcacgct cttttggaca accacccaac
      721 atgttgtgct tgtcagctat ataacgtatg atggccatag actgtgttaa tttaacatca
      781 ccatcaatat aataaggaag attgggaaac tcc
//
