LOCUS       sequence-164802-         971 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway-compatible zebrafish gfap promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Fowler DK, Stewart S, Seredick S, Eisen JS, Stankunas K, Washbourne 
            P
  TITLE     A MultiSite Gateway Toolkit for Rapid Cloning of Vertebrate 
            Expression Constructs with Diverse Research Applications.
  JOURNAL   PLoS One. 2016 Aug 8;11(8):e0159277. doi: 
            10.1371/journal.pone.0159277. eCollection 2016.
  PUBMED    27500400
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-8"; volume: "11(8):e0159277. doi"; pages: " 
            10.1371/journal.pone.0159277. eCollection 2016"
COMMENT     Sequence Label: p5E-gfap
FEATURES             Location/Qualifiers
     source          1..971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    7..102
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     104..123
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        104..122
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 agctccaaat aatgatttta ttttgactga tagtgacctg ttcgttgcaa caaattgata
       61 agcaatgctt ttttataatg ccaactttgt atagaaaagt tggtaatacg actcactata
      121 gggcgaattg ggtaccgggc cccccctcga ggtaaggact gaggtgatgc acagtgttgg
      181 gtatgttact ttcaataagt tattctttgc taattattag tcatttgaat tgtattgaaa
      241 ttacattttt agttactttg cctaaaaaaa aacttgataa gtaatcgaat tacatacttg
      301 aagtccacat aaatctcatt cattcattcc ttctcttttc gacttagtcc ctttattaat
      361 caggggtttc tacagcggaa cgaacctcat atgtttcaat ttagtgacta ttttttataa
      421 attattgaca atgtttttgt tgcttgttca aactacttat ttaaaatgag ctgaaactgc
      481 acaattattg atatttcatt ggaacagctt aatttttttt atgtttaatc cacttaaata
      541 aaattccaat tatataatat tccaattata aagttactta atcaatttgt attaggacaa
      601 tatgaatgaa ttgtgtggaa ccctgcattt tttacagtga acctttcctt gtttcttaaa
      661 catgggtgat tttcggagaa ataaaatttg acaatatctg aaatgtaaaa agaaaatatt
      721 cagttactct gcctttctaa atgcatagtt tatattatca tcagagttgt aaagtaacta
      781 gttacaaata ctcaaattac tgtaattgag tagttttttt caggaattgt aatttactaa
      841 gtagctaaaa aatgtgtact tttactttcc cttgagtaca ttattaatgc tgtaacggta
      901 cttttactcc actactttcc ttcagcctgc actcactact ttgttttatc ttgtctatga
      961 ggattagaaa a
//
