LOCUS       sequence-164805-         963 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway-compatible zebrafish gfap promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Fowler DK, Stewart S, Seredick S, Eisen JS, Stankunas K, Washbourne 
            P
  TITLE     A MultiSite Gateway Toolkit for Rapid Cloning of Vertebrate 
            Expression Constructs with Diverse Research Applications.
  JOURNAL   PLoS One. 2016 Aug 8;11(8):e0159277. doi: 
            10.1371/journal.pone.0159277. eCollection 2016.
  PUBMED    27500400
REFERENCE   2  (bases 1 to 963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-8"; volume: "11(8):e0159277. doi"; pages: " 
            10.1371/journal.pone.0159277. eCollection 2016"
COMMENT     Sequence Label: p5E-gfap
FEATURES             Location/Qualifiers
     source          1..963
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(66..189)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        190..208
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     245..261
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     245..261
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 gcggccgccc tgcagctcta gagctcgaat tctacaggtc actaatacca tctaagtagt
       61 tggttcatag tgactgcata tgttgtgttt tacagtatta tgtagtctgt tttttatgca
      121 aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca acttttttgt
      181 acaaacttga attaaccctc actaaaggga acaaaagctg gagctccacc gcggtggcgg
      241 ccgctctaga actagtggat cccgggcccg cggtaccgtc gacgaggaac gctgggactc
      301 catggtggag gagaatgaga tcgagtgcgg gtgaactctg ggtttttata gagttgatgt
      361 ggggggtgaa agagagaggg ggggtctgat ggggagggca aaggccgagg gggtgtttgt
      421 gagcccgcaa ttgtttgtgc ttgtccagcc agtctcctga ctgcctcttt tcacagtgag
      481 ccagagaaaa tcctattcat accctgctgg aacacagaaa tagagagact gagatggcat
      541 cagcactgat attggacttt aattctctct tacctcatgc taaagagttg gtttgcagat
      601 cttctgagtt actccggagt tgctctgctt tctcagtcac agttttgttc tgtctgggag
      661 tttaatggtt tcagggaaca caggaggtga ttacgtaatt cttcattatt aaaagaaact
      721 ggcgctccct cttttgtgct gatctgagaa catcacacac atgctcttca gcagcactga
      781 tttatatcgg tttagcagaa aaatcatagg aattgatttg tttacttgaa ctcatgctgg
      841 ttaaatgtta aaggggttat tcatccaata aacattttgc ttgtgtgctc catatctgag
      901 cacaatttta acatgcaaag aaacataatt aaagaaaact ttccttataa agctactttg
      961 aga
//
