LOCUS       sequence-164814-         980 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mutant GST-CD-ATL (Cbx7) fusion proteins in E. coli. Cbx7 
            CD-ATL region, amino acids 1–84..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 980)
  TITLE     pTRIPZ-MSC1-Halotag-Cbx2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pGEX-6P-1-GST-CD-ATL (Cbx7)
FEATURES             Location/Qualifiers
     source          1..980
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     120..142
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(482..505)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(532..554)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga
       61 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
      121 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagatcgt
      181 cagtcagtca cgatgcggcc gctcgagctc acagccgctg cagcagaagc cgcttgggtt
      241 tcggacctct cttcctatac cccgatgctc ggtctctctc ctccttctcc tcgtaggcca
      301 tgacgaggcg ggggtccaag atgtgctctt ctggctccca cgtgctgtac tttgggggcc
      361 atcctttcca cttcaccaga tactcgactt tacccttccg cacgcgcttc ttccggatgc
      421 tctccacggc gaacacctgc tcgccgatgg ctgacagctc catgaattcc ggggatccca
      481 ggggcccctg gaacagaact tccagatccg attttggagg atggtcgcca ccaccaaacg
      541 tggcttgcca gccctgcaaa ggccatgcta tatacttgct ggatttcaag tacttatcaa
      601 tttgtgggat agcttcaata cgttttttaa aacaaactaa ttttgggaac gcatccaggc
      661 acattgggtc catgtataaa acaacatcaa gagcgtcata caacatgaag tcaggatggg
      721 ttacatgatc accatttaaa tatgttttat gacataaacg atcttcgaac attttcagca
      781 tttcaggtag cttgctaaga aaatcaactt tgagagtttc aaagtcttta ctatatgcaa
      841 ttctcgaaac accgtatcta atatccaaaa ccgctccttc aagcattgaa atctctgcac
      901 gctcttttgg acaaccaccc aacatgttgt gcttgtcagc tatataacgt atgatggcca
      961 tagactgtgt taatttaaca
//
