LOCUS       sequence-164821-         957 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mutant GST-CD-ATLm (Cbx7) fusion proteins in E. coli. Cbx7
            amino acids 1-84 with mutation of RKR70-72, K75, and KR77-78 to 
            AGA70-72, A75, and AG77-78..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 957)
  TITLE     pTRIPZ-MSC1-Halotag-Cbx2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pGEX-6P-1-GST-CD-ATLm(Cbx7)
FEATURES             Location/Qualifiers
     source          1..957
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     121..143
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(483..506)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(533..555)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg
       61 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
      121 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagatcg
      181 tcagtcagtc acgatgcggc cgctcgagct cacagccgct gcagcagaag gcctgcgggt
      241 gccggacctg cgcctgcata ccccgatgct cggtctctct cctccttctc ctcgtaggcc
      301 atgacgaggc gggggtccaa gatgtgctct tctggctccc acgtgctgta ctttgggggc
      361 catcctttcc acttcaccag atactcgact ttacccttcc gcacgcgctt cttccggatg
      421 ctctccacgg cgaacacctg ctcgccgatg gctgacagct ccatgaattc cggggatccc
      481 aggggcccct ggaacagaac ttccagatcc gattttggag gatggtcgcc accaccaaac
      541 gtggcttgcc agccctgcaa aggccatgct atatacttgc tggatttcaa gtacttatca
      601 atttgtggga tagcttcaat acgtttttta aaacaaacta attttgggaa cgcatccagg
      661 cacattgggt ccatgtataa aacaacatca agagcgtcat acaacatgaa gtcaggatgg
      721 gttacatgat caccatttaa atatgtttta tgacataaac gatcttcgaa cattttcagc
      781 atttcaggta gcttgctaag aaaatcaact ttgagagttt caaagtcttt actatatgca
      841 attctcgaaa caccgtatct aatatccaaa accgctcctt caagcattga aatctctgca
      901 cgctcttttg gacaaccacc caacatgttg tgcttgtcag ctatataacg tatgatg
//
