LOCUS       sequence-164829-         963 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway-compatible Brainbow-1.0 with fluorophores 
            tdTomato-myc/EGFP/E2Crimson-HA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Fowler DK, Stewart S, Seredick S, Eisen JS, Stankunas K, Washbourne 
            P
  TITLE     A MultiSite Gateway Toolkit for Rapid Cloning of Vertebrate 
            Expression Constructs with Diverse Research Applications.
  JOURNAL   PLoS One. 2016 Aug 8;11(8):e0159277. doi: 
            10.1371/journal.pone.0159277. eCollection 2016.
  PUBMED    27500400
REFERENCE   2  (bases 1 to 963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-8"; volume: "11(8):e0159277. doi"; pages: " 
            10.1371/journal.pone.0159277. eCollection 2016"
COMMENT     Sequence Label: pME-BrainbowTEC
FEATURES             Location/Qualifiers
     source          1..963
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    151..272
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(188..207)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     242..261
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(279..312)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      328..337
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     732..751
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tggacgagct gtacaagtag ggccggccag cggccgcgac tctagatcat aatcagccat
       61 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
      121 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
      181 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
      241 tgtggtttgt ccaaactcat caatgtatct taatcgagat aacttcgtat agcatacatt
      301 atacgaagtt ataccgttaa ttaagccgcc accatggata gcactgagaa cgtcatcaag
      361 cccttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
      421 gagggcgtgg gcgagggcaa gccctacgag ggcacccaga ccgccaagct gcaagtgacc
      481 aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttctt ctacggctcc
      541 aaggcgtaca tcaagcaccc cgccgacatc cccgactacc tcaagcagtc cttccccgag
      601 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
      661 gactcctccc tgcaggacgg caccctcatc taccacgtga agttcatcgg cgtgaacttc
      721 ccctccgacg gccccgtaat gcagaagaag actctgggct gggagccctc cactgagcgc
      781 aactaccccc gcgacggcgt gctgaagggc gagaaccaca tggcgctgaa gctgaagggc
      841 ggcggccact acctgtgtga gttcaagtcc atctacatgg ccaagaagcc cgtgaagctg
      901 cccggctacc actacgtgga ctacaagctc gacatcacct cccacaacga ggactacacc
      961 gtg
//
