LOCUS       sequence-164830-         530 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway-compatible FlEx cassette containing two pairs of 
            heterotypic, antiparallel loxP-type recombination sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Fowler DK, Stewart S, Seredick S, Eisen JS, Stankunas K, Washbourne 
            P
  TITLE     A MultiSite Gateway Toolkit for Rapid Cloning of Vertebrate 
            Expression Constructs with Diverse Research Applications.
  JOURNAL   PLoS One. 2016 Aug 8;11(8):e0159277. doi: 
            10.1371/journal.pone.0159277. eCollection 2016.
  PUBMED    27500400
REFERENCE   2  (bases 1 to 530)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-8"; volume: "11(8):e0159277. doi"; pages: " 
            10.1371/journal.pone.0159277. eCollection 2016"
COMMENT     Sequence Label: pME-FlEx switch
FEATURES             Location/Qualifiers
     source          1..530
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    3..102
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    116..149
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    212..245
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(281..314)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(385..418)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(431..530)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 cccaaataat gattttattt tgactgatag tgacctgttc gttgcaacaa attgatgagc
       61 aatgcttttt tataatgcca actttgtaca aaaaagcagg cttcggcgcg ccgtcataac
      121 ttcgtataaa gtatcctata cgaagttata tcaaaatagg aagaccaatg cttcaccatc
      181 gacccgaatt gccaagcatc accatcgacc cataacttcg tataatgtat gctatacgaa
      241 gttatatgcg gccgcatcga tcgatggccg aggcggccat ataacttcgt ataggatact
      301 ttatacgaag ttatcattgg gattcttcct attttgatcc aagcatcacc atcgaccctc
      361 tagtccggat ctcaccatcg acccataact tcgtatagca tacattatac gaagttatgt
      421 cttaattaag acccagcttt cttgtacaaa gttggcatta taagaaagca ttgcttatca
      481 atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg
//
