LOCUS       sequence-164832-         970 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  csd1, construct: 54-312, pET21a(+) C-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 970)
  AUTHORS   An DR, Im HN, Jang JY, Kim HS, Kim J, Yoon HJ, Hesek D, Lee M, 
            Mobashery S, Kim SJ, Suh SW
  TITLE     Structural Basis of the Heterodimer Formation between Cell 
            Shape-Determining Proteins Csd1 and Csd2 from Helicobacter pylori.
  JOURNAL   PLoS One. 2016 Oct 6;11(10):e0164243. doi: 
            10.1371/journal.pone.0164243. eCollection 2016.
  PUBMED    27711177
REFERENCE   2  (bases 1 to 970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-10-6"; volume: "11(10):e0164243. doi"; pages: " 
            10.1371/journal.pone.0164243. eCollection 2016"
COMMENT     Sequence Label: csd1_54-312/pET21a(+)
FEATURES             Location/Qualifiers
     source          1..970
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             835..852
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(905..923)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      919..966
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ttcccctcta gaaataattt tgtttaactt taagaaggag atatacatat ggagattaaa
       61 aacattgaca agcagcatgc tctaatcact aaagaatttg agaaaaaaaa agagacgaat
      121 gaaaagcttt ccttgcaaat ggatgaattt ttagacgatt tgcagctttc aggggaacgc
      181 atcaacgatt tagaagaagt ggtgggagtg aataggcctg aagaagaaaa agaagagggc
      241 aatttttcca gtcgtttgga tgtcgctggg attaccgggc ttcaaaaaag ctttatcatg
      301 cgccttatcc ctaatgacta cccgctagaa tcctatcggc gcgtttcagc cgcctttaat
      361 aaaagaatcc accctatttt gcatgtgctg cacaaccata ccgggcttga tttaagcacc
      421 gctattaaca cccctgtgta tgcgagcgcg agcggggtag tagggttagc gagcaagggg
      481 tggaatgggg ggtatgggaa tttgattaaa gttttccacc cttttggttt taaaacctac
      541 tacgcccatt tgaataaaat cgtcgtaaaa acgggcgaat ttgtcaaaaa agggcagttg
      601 attgggtata gtggtaatac agggatgagc acagggccgc atttgcatta tgaagtgcgt
      661 tttttagatc aacccataaa ccccatgagt ttcaccaaat ggaacatgaa agattttgaa
      721 gaagttttca ataaagaaag gagcatcaga tggcaatctt tgataacaat aataaatcgg
      781 ctaatgcaaa aacaggacca gcgactatca tcgctcaagg cacaaaaact cgagcaccac
      841 caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct
      901 gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt
      961 tttttgctga
//
