LOCUS       sequence-164833-         962 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  csd1, construct: 54-312, pET21a(+) C-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 962)
  AUTHORS   An DR, Im HN, Jang JY, Kim HS, Kim J, Yoon HJ, Hesek D, Lee M, 
            Mobashery S, Kim SJ, Suh SW
  TITLE     Structural Basis of the Heterodimer Formation between Cell 
            Shape-Determining Proteins Csd1 and Csd2 from Helicobacter pylori.
  JOURNAL   PLoS One. 2016 Oct 6;11(10):e0164243. doi: 
            10.1371/journal.pone.0164243. eCollection 2016.
  PUBMED    27711177
REFERENCE   2  (bases 1 to 962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-10-6"; volume: "11(10):e0164243. doi"; pages: " 
            10.1371/journal.pone.0164243. eCollection 2016"
COMMENT     Sequence Label: csd1_54-312/pET21a(+)
FEATURES             Location/Qualifiers
     source          1..962
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             846..868
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    883..907
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(907..926)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(908..926)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtt
       61 tttgtgcctt gagcgatgat agtcgctggt cctgtttttg cattagccga tttattattg
      121 ttatcaaaga ttgccatctg atgctccttt ctttattgaa aacttcttca aaatctttca
      181 tgttccattt ggtgaaactc atggggttta tgggttgatc taaaaaacgc acttcataat
      241 gcaaatgcgg ccctgtgctc atccctgtat taccactata cccaatcaac tgcccttttt
      301 tgacaaattc gcccgttttt acgacgattt tattcaaatg ggcgtagtag gttttaaaac
      361 caaaagggtg gaaaacttta atcaaattcc catacccccc attccacccc ttgctcgcta
      421 accctactac cccgctcgcg ctcgcataca caggggtgtt aatagcggtg cttaaatcaa
      481 gcccggtatg gttgtgcagc acatgcaaaa tagggtggat tcttttatta aaggcggctg
      541 aaacgcgccg ataggattct agcgggtagt cattagggat aaggcgcatg ataaagcttt
      601 tttgaagccc ggtaatccca gcgacatcca aacgactgga aaaattgccc tcttcttttt
      661 cttcttcagg cctattcact cccaccactt cttctaaatc gttgatgcgt tcccctgaaa
      721 gctgcaaatc gtctaaaaat tcatccattt gcaaggaaag cttttcattc gtctcttttt
      781 ttttctcaaa ttctttagtg attagagcat gctgcttgtc aatgttttta atctccatat
      841 gtatatctcc ttcttaaagt taaacaaaat tatttctaga ggggaattgt tatccgctca
      901 caattcccct atagtgagtc gtattaattt cgcgggatcg agatctcgat cctctacgcc
      961 gg
//
