LOCUS       sequence-164834-         512 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway-compatible FlEx cassette containing two pairs of 
            heterotypic, antiparallel loxP-type recombination sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Fowler DK, Stewart S, Seredick S, Eisen JS, Stankunas K, Washbourne 
            P
  TITLE     A MultiSite Gateway Toolkit for Rapid Cloning of Vertebrate 
            Expression Constructs with Diverse Research Applications.
  JOURNAL   PLoS One. 2016 Aug 8;11(8):e0159277. doi: 
            10.1371/journal.pone.0159277. eCollection 2016.
  PUBMED    27500400
REFERENCE   2  (bases 1 to 512)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-8"; volume: "11(8):e0159277. doi"; pages: " 
            10.1371/journal.pone.0159277. eCollection 2016"
COMMENT     Sequence Label: pME-FlEx switch
FEATURES             Location/Qualifiers
     source          1..512
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    60..84
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    97..130
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    201..234
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(270..303)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(366..399)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(413..512)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 tttgactgat agtgacctgt tcgttgcaac aaattgataa gcaatgcttt cttataatgc
       61 caactttgta caagaaagct gggtcttaat taagacataa cttcgtataa tgtatgctat
      121 acgaagttat gggtcgatgg tgagatccgg actagagggt cgatggtgat gcttggatca
      181 aaataggaag aatcccaatg ataacttcgt ataaagtatc ctatacgaag ttatatggcc
      241 gcctcggcca tcgatcgatg cggccgcata taacttcgta tagcatacat tatacgaagt
      301 tatgggtcga tggtgatgct tggcaattcg ggtcgatggt gaagcattgg tcttcctatt
      361 ttgatataac ttcgtatagg atactttata cgaagttatg acggcgcgcc gaagcctgct
      421 tttttgtaca aagttggcat tataaaaaag cattgctcat caatttgttg caacgaacag
      481 gtcactatca gtcaaaataa aatcattatt tg
//
