LOCUS       sequence-164835-        6147 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  csd1, construct: 54-312, pET21a(+) C-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6147)
  AUTHORS   An DR, Im HN, Jang JY, Kim HS, Kim J, Yoon HJ, Hesek D, Lee M, 
            Mobashery S, Kim SJ, Suh SW
  TITLE     Structural Basis of the Heterodimer Formation between Cell 
            Shape-Determining Proteins Csd1 and Csd2 from Helicobacter pylori.
  JOURNAL   PLoS One. 2016 Oct 6;11(10):e0164243. doi: 
            10.1371/journal.pone.0164243. eCollection 2016.
  PUBMED    27711177
REFERENCE   2  (bases 1 to 6147)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-10-6"; volume: "11(10):e0164243. doi"; pages: " 
            10.1371/journal.pone.0164243. eCollection 2016"
COMMENT     Sequence Label: csd1_54-312/pET21a(+)
FEATURES             Location/Qualifiers
     source          1..6147
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      83..671
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     572..591
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     825..842
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    857..999
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(925..944)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1085..1107
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(1101..1292)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(2101..3183)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     3145..3164
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(3184..3261)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     3467..3486
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     3570..3589
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3570..3588
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3589..3613
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             3628..3650
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             4444..4461
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4514..4532)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      4528..4575
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      4612..5067
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4699..4718)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4909..4930
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5094..5198
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5199..5267,5268..6059)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5417..5436)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
       61 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
      121 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
      181 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
      241 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
      301 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
      361 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
      421 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
      481 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
      541 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
      601 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
      661 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
      721 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
      781 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
      841 aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
      901 accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
      961 tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc
     1021 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     1081 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag
     1141 ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc
     1201 gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc
     1261 atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg
     1321 ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat
     1381 gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg
     1441 cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt
     1501 gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc
     1561 gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt
     1621 gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat
     1681 tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc
     1741 acgatcatgc gcacccgtgg ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa
     1801 cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc
     1861 gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc
     1921 cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg
     1981 gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag
     2041 ggcatcggtc gagatcccgg tgcctaatga gtgagctaac ttacattaat tgcgttgcgc
     2101 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     2161 cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt tcaccagtga
     2221 gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc
     2281 cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata
     2341 acatgagctg tcttcggtat cgtcgtatcc cactaccgag atatccgcac caacgcgcag
     2401 cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat
     2461 cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac cggacatggc
     2521 actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga gatatttatg
     2581 ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta acagcgcgat
     2641 ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt cttcatggga
     2701 gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg ccggaacatt
     2761 agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag
     2821 cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt cgacgccgct
     2881 tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag atttaatcgc
     2941 cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc caatcagcaa
     3001 cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca gctccgccat
     3061 cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg
     3121 ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg ttactggttt
     3181 cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt
     3241 tttgcgccat tcgatggtgt ccgggatctc gacgctctcc cttatgcgac tcctgcatta
     3301 ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat
     3361 gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga
     3421 aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga
     3481 tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt
     3541 agaggatcga gatctcgatc ccgcgaaatt aatacgactc actatagggg aattgtgagc
     3601 ggataacaat tcccctctag aaataatttt gtttaacttt aagaaggaga tatacatatg
     3661 gagattaaaa acattgacaa gcagcatgct ctaatcacta aagaatttga gaaaaaaaaa
     3721 gagacgaatg aaaagctttc cttgcaaatg gatgaatttt tagacgattt gcagctttca
     3781 ggggaacgca tcaacgattt agaagaagtg gtgggagtga ataggcctga agaagaaaaa
     3841 gaagagggca atttttccag tcgtttggat gtcgctggga ttaccgggct tcaaaaaagc
     3901 tttatcatgc gccttatccc taatgactac ccgctagaat cctatcggcg cgtttcagcc
     3961 gcctttaata aaagaatcca ccctattttg catgtgctgc acaaccatac cgggcttgat
     4021 ttaagcaccg ctattaacac ccctgtgtat gcgagcgcga gcggggtagt agggttagcg
     4081 agcaaggggt ggaatggggg gtatgggaat ttgattaaag ttttccaccc ttttggtttt
     4141 aaaacctact acgcccattt gaataaaatc gtcgtaaaaa cgggcgaatt tgtcaaaaaa
     4201 gggcagttga ttgggtatag tggtaataca gggatgagca cagggccgca tttgcattat
     4261 gaagtgcgtt ttttagatca acccataaac cccatgagtt tcaccaaatg gaacatgaaa
     4321 gattttgaag aagttttcaa taaagaaagg agcatcagat ggcaatcttt gataacaata
     4381 ataaatcggc taatgcaaaa acaggaccag cgactatcat cgctcaaggc acaaaaactc
     4441 gagcaccacc accaccacca ctgagatccg gctgctaaca aagcccgaaa ggaagctgag
     4501 ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc
     4561 ttgaggggtt ttttgctgaa aggaggaact atatccggat tggcgaatgg gacgcgccct
     4621 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     4681 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     4741 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     4801 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     4861 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     4921 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     4981 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     5041 ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa
     5101 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     5161 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     5221 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     5281 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     5341 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     5401 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     5461 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     5521 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     5581 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     5641 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     5701 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt
     5761 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     5821 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     5881 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     5941 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     6001 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     6061 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     6121 aaaggatcta ggtgaagatc ctttttg
//
