LOCUS       sequence-164840-        1020 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  csd2, construct:140-251, pET21a(+) C-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   An DR, Im HN, Jang JY, Kim HS, Kim J, Yoon HJ, Hesek D, Lee M, 
            Mobashery S, Kim SJ, Suh SW
  TITLE     Structural Basis of the Heterodimer Formation between Cell 
            Shape-Determining Proteins Csd1 and Csd2 from Helicobacter pylori.
  JOURNAL   PLoS One. 2016 Oct 6;11(10):e0164243. doi: 
            10.1371/journal.pone.0164243. eCollection 2016.
  PUBMED    27711177
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-10-6"; volume: "11(10):e0164243. doi"; pages: " 
            10.1371/journal.pone.0164243. eCollection 2016"
COMMENT     Sequence Label: csd2_140-251/pET21a(+)
FEATURES             Location/Qualifiers
     source          1..1020
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             388..405
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(458..476)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      472..519
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      556..1011
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(643..662)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     853..874
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ccctctagaa ataattttgt ttaactttaa gaaggagata tacatatgcc cataaaaact
       61 tatagcgcta tcaaacccac caaagaaagg aaccacccca ttaaaaaaat taagggcgtt
      121 gaatccggga ttgattttat cgcgccatta aacacgcctg tttatgcgag cgctgatggg
      181 attgtggatt ttgtgaagac caattctaat gtggggtatg ggaacttggt gcgcattgaa
      241 catgcgtttg gttttagctc catttacacg cacttagatc atgtcaatgt gcagcccaaa
      301 agcttcatcc aaaaagggca gttgattggc tatagcggga agagcggtaa tagcggcggc
      361 gaaaaattgc attatgaagt gctcgagcac caccaccacc accactgaga tccggctgct
      421 aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa
      481 ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc
      541 ggattggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
      601 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
      661 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
      721 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
      781 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
      841 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
      901 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
      961 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac
//
