LOCUS       sequence-164842-         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  csd2, construct:140-251, pET21a(+) C-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  AUTHORS   An DR, Im HN, Jang JY, Kim HS, Kim J, Yoon HJ, Hesek D, Lee M, 
            Mobashery S, Kim SJ, Suh SW
  TITLE     Structural Basis of the Heterodimer Formation between Cell 
            Shape-Determining Proteins Csd1 and Csd2 from Helicobacter pylori.
  JOURNAL   PLoS One. 2016 Oct 6;11(10):e0164243. doi: 
            10.1371/journal.pone.0164243. eCollection 2016.
  PUBMED    27711177
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-10-6"; volume: "11(10):e0164243. doi"; pages: " 
            10.1371/journal.pone.0164243. eCollection 2016"
COMMENT     Sequence Label: csd2_140-251/pET21a(+)
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             402..424
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    439..463
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(463..482)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(464..482)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(566..585)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        791..868
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     complement(888..907)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagca
       61 cttcataatg caatttttcg ccgccgctat taccgctctt cccgctatag ccaatcaact
      121 gccctttttg gatgaagctt ttgggctgca cattgacatg atctaagtgc gtgtaaatgg
      181 agctaaaacc aaacgcatgt tcaatgcgca ccaagttccc ataccccaca ttagaattgg
      241 tcttcacaaa atccacaatc ccatcagcgc tcgcataaac aggcgtgttt aatggcgcga
      301 taaaatcaat cccggattca acgcccttaa tttttttaat ggggtggttc ctttctttgg
      361 tgggtttgat agcgctataa gtttttatgg gcatatgtat atctccttct taaagttaaa
      421 caaaattatt tctagagggg aattgttatc cgctcacaat tcccctatag tgagtcgtat
      481 taatttcgcg ggatcgagat ctcgatcctc tacgccggac gcatcgtggc cggcatcacc
      541 ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg
      601 gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg
      661 gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc
      721 aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt
      781 cgagatcccg gacaccatcg aatggcgcaa aacctttcgc ggtatggcat gatagcgccc
      841 ggaagagagt caattcaggg tggtgaatgt gaaaccagta acgttatacg atgtcgcaga
      901 gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca
//
