LOCUS       sequence-164859-         618 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral Sp dCas9-p300-P2A-PuroR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Klann TS, Black JB, Chellappan M, Safi A, Song L, Hilton IB, 
            Crawford GE, Reddy TE, Gersbach CA
  TITLE     CRISPR-Cas9 epigenome editing enables high-throughput screening for 
            functional regulatory elements in the human genome.
  JOURNAL   Nat Biotechnol. 2017 Apr 3. doi: 10.1038/nbt.3853.
  PUBMED    28369033
REFERENCE   2  (bases 1 to 618)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2017 Apr 3. doi: 10.1038/nbt.3853."
COMMENT     Sequence Label: pLV-dCas9-p300-P2A-PuroR
FEATURES             Location/Qualifiers
     source          1..618
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(122..142)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 attttgtaat ccagaggttg attgtcgact taacgcgttc aggcaccggg cttgcgggtc
       61 atgcaccagg tgcgcggtcc ttcgggcacc tcgacgtcgg cggtgacggt gaagccgagc
      121 cgctcgtaga aggggaggtt gcggggcgcg gaggtctcca ggaaggcggg caccccggcg
      181 cgctcggccg cctccactcc ggggagcacg acggcgctgc ccagaccctt gccctggtgg
      241 tcgggcgaga ctccgacggt ggccaggaac cacgcgggct ccttgggccg gtgcggcgcc
      301 aggaggcctt ccatctgttg ctgcgcggcc agccgggaac cgctcaactc ggccatgcgc
      361 gggccgatct cggcgaacac cgcccccgct tcgacgctct ccggcgtggt ccagaccgcc
      421 accgcggcgc cgtcgtccgc gacccacacc ttgccgatgt cgagcccgac gcgcgtgagg
      481 aagagttctt gcagctcggt gacccgctcg atgtggcggt ccggatcgac ggtgtggcgc
      541 gtggcggggt agtcggcgaa cgcggcggcg agggtgcgta cggccctggg gacgtcgtcg
      601 cgggtggcga ggcgcacc
//
