LOCUS       sequence-164862-        1005 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses m23 peptidase from Campylobacter jejuni.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1005)
  TITLE     C.jejuni_peptidase m23 family
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET21a(+)_C.jejuni m23 peptidase
FEATURES             Location/Qualifiers
     source          1..1005
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             828..850
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    865..889
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(889..908)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(890..908)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtt
       61 gaaaaatagc attaaatttg gatacaaaat ccaaaggatc aacttgtttg cctccagcta
      121 aaattccaaa atgcaaatgc ggcccactta ccctaccact agccccacta agtcctataa
      181 gttcaccttt ttttatcttt tgtcctactt taacatcgat tttagaaaga tgataatatt
      241 gtgaataaat tccaaaacca tgatcaatga ctactgaatt tcctgcgaaa taacgatctt
      301 ttgcaatttt tactacacct gaattggctg cataaatagg cgttcctgtg gcggctctaa
      361 agtccgttcc actatgataa cttgctactt tttcattaaa agttcttgct ttgccaaaat
      421 cacttgtgat aaaagaattt aaaggtatat taaaagcacc gttaaataaa gcttttggag
      481 tataagaact ataaatcgca ttggcttctt ttaattcttt agcgatgcgt tcttgtatgg
      541 tttttggtgg aaaaatcttt ttgttttcaa cttgcaattt ttcactttta taattacctt
      601 ctaaagtttt tatgaaaatt tcttcttttt tatctttata aaatgcgata agttttgtat
      661 tttgcggggg atttttgtaa ggtaaggaaa aaatagctag aattttttct tgatttttag
      721 gatgtgcaaa ggtgggaata tttttatcat tatttttcaa agataaaaaa tctttcttat
      781 ctagttccaa aaataaagct tgacctttga tgagttccat atgtatatct ccttcttaaa
      841 gttaaacaaa attatttcta gaggggaatt gttatccgct cacaattccc ctatagtgag
      901 tcgtattaat ttcgcgggat cgagatctcg atcctctacg ccggacgcat cgtggccggc
      961 atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgac
//
