LOCUS       sequence-164870-        4320 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing FH , part of the Target Accelerator 
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4320)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_FH_WT
FEATURES             Location/Qualifiers
     source          1..4320
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1720..1819)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1836..1855)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1837..1855)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1860..1876)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1860..1876)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2307..3098
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3191..3779
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3680..3699
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3933..3950
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4109..4136)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4228..4314)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcat gtaccgagca cttcggctcc tcgcgcgctc gcgtcccctc gtgcgggctc
      241 cagccgcagc cttagcttcg gctcccggct tgggtggcgc ggccgtgccc tcgttttggc
      301 ctccgaacgc ggctcgaatg gcaagccaaa attccttccg gatagaatat gatacctttg
      361 gtgaactaaa ggtgccaaat gataagtatt atggcgccca gaccgtgaga tctacgatga
      421 actttaagat tggaggtgtg acagaacgca tgccaacccc agttattaaa gcttttggca
      481 tcttgaagcg agcggccgct gaagtaaacc aggattatgg tcttgatcca aagattgcta
      541 atgcaataat gaaggcagca gatgaggtag ctgaaggtaa attaaatgat cattttcctc
      601 tcgtggtatg gcagactgga tcaggaactc agacaaatat gaatgtaaat gaagtcatta
      661 gcaatagagc aattgaaatg ttaggaggtg aacttggcag caagatacct gtgcatccca
      721 acgatcatgt taataaaagc cagagctcaa atgatacttt tcccacagca atgcacattg
      781 ctgctgcaat agaagttcat gaagtactgt taccaggact acagaagtta catgatgctc
      841 ttgatgcaaa atccaaagag tttgcacaga tcatcaagat tggacgtact catactcagg
      901 atgctgttcc acttactctt gggcaggaat ttagtggtta tgttcaacaa gtaaaatatg
      961 caatgacaag aataaaagct gccatgccaa gaatctatga gctcgcagct ggaggcactg
     1021 ctgttggtac aggtttaaat actagaattg gctttgcaga aaaggttgct gcaaaagtgg
     1081 ctgcacttac aggcttgcct tttgtcactg ctccgaataa atttgaagct ctggctgctc
     1141 atgacgctct ggttgagctc agtggagcca tgaacactac tgcctgcagt ctgatgaaga
     1201 tagcaaatga tattcgattt ttgggttctg gtcctcggtc aggtctggga gaattgatct
     1261 tgcctgaaaa tgaaccagga agcagtatca tgccaggcaa ggtgaaccct actcagtgtg
     1321 aagcaatgac catggttgca gcccaagtca tggggaacca tgttgctgtc actgtcggag
     1381 gcagcaatgg acattttgag ttgaatgttt tcaagccaat gatgattaaa aatgtgttac
     1441 actcagccag gctgctgggg gatgcttcag tttcctttac agaaaactgc gtggtgggaa
     1501 tccaggccaa tacagaaagg atcaacaagc tgatgaatga gtctctaatg ttggtgacag
     1561 ctctcaatcc tcatataggg tatgacaagg cagcaaagat tgctaagaca gcacacaaaa
     1621 atggatcaac cttaaaggaa actgctatcg aacttggcta tctcacagca gagcagtttg
     1681 acgaatgggt aaaacctaag gacatgctgg gtccaaagtg cccaactttc ttgtacaaag
     1741 ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc
     1801 aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg tattacatgg
     1861 tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac
     1921 aagataaaaa tatatcatca tgcctcctct agaccagcca ggacagaaat gcctcgactt
     1981 cgctgctacc caaggttgcc gggtgacgca caccgtggaa acggatgaag gcacgaaccc
     2041 agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca
     2101 actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg
     2161 cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc
     2221 gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca
     2281 gtcgccctaa aacaaagtta aacattatga gggaagcggt gatcgccgaa gtatcgactc
     2341 aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac
     2401 atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg
     2461 ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg
     2521 aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg
     2581 tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat
     2641 ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg
     2701 ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg
     2761 aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc
     2821 tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc
     2881 gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg
     2941 caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc
     3001 ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact
     3061 acgtgaaagg cgagatcacc aaggtagtcg gcaaataacc ctcgagccac ccatgaccaa
     3121 aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca
     3181 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     3241 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     3301 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     3361 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     3421 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     3481 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     3541 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     3601 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     3661 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     3721 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     3781 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     3841 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     3901 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     3961 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     4021 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg
     4081 ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt
     4141 agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt
     4201 cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa
     4261 acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta tttgatgcct
//
