LOCUS       sequence-164871-         610 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentivirus vector to express guideRNA and dCas9 with puro resistant 
            gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Murakami K, Sasaki H, Nishiyama A, Kurotaki D, Kawase W, Ban T, 
            Nakabayashi J, Kanzaki S, Sekita Y, Nakajima H, Ozato K, Kimura T, 
            Tamura T
  TITLE     A RUNX-CBFbeta-driven enhancer directs the Irf8 dose-dependent 
            lineage choice between DCs and monocytes.
  JOURNAL   Nat Immunol. 2021 Mar;22(3):301-311. doi: 
            10.1038/s41590-021-00871-y. Epub 2021 Feb 18.
  PUBMED    33603226
REFERENCE   2  (bases 1 to 610)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41590-021-00871-y"; journalName: "Nat Immunol"; date: 
            "2021-03"; volume: "22"; issue: "3"; pages: "301-311"
COMMENT     Sequence Label: CSII-U6-gRNA-CBh-3xFLAG-PA-dCas9-P2A-Puro
FEATURES             Location/Qualifiers
     source          1..610
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        33..108
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        205..490
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 aaggacgaaa caccgggtct tcgagaagac ctgttttaga gctagaaata gcaagttaaa
       61 ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt ttttgtttta
      121 gagctagaaa tagcaagtta aaataaggct agtccgtttt tagcgcgtgc gccaattctg
      181 cagacaaatg gctctagagg tacccgttac ataacttacg gtaaatggcc cgcctggctg
      241 accgcccaac gacccccgcc cattgacgtc aatagtaacg ccaataggga ctttccattg
      301 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      361 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattgtgc
      421 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      481 tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc
      541 cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg
      601 gggggggggg
//
