LOCUS       sequence-164873-         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentivirus vector to express guideRNA and dCas9 with puro resistant 
            gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Murakami K, Sasaki H, Nishiyama A, Kurotaki D, Kawase W, Ban T, 
            Nakabayashi J, Kanzaki S, Sekita Y, Nakajima H, Ozato K, Kimura T, 
            Tamura T
  TITLE     A RUNX-CBFbeta-driven enhancer directs the Irf8 dose-dependent 
            lineage choice between DCs and monocytes.
  JOURNAL   Nat Immunol. 2021 Mar;22(3):301-311. doi: 
            10.1038/s41590-021-00871-y. Epub 2021 Feb 18.
  PUBMED    33603226
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41590-021-00871-y"; journalName: "Nat Immunol"; date: 
            "2021-03"; volume: "22"; issue: "3"; pages: "301-311"
COMMENT     Sequence Label: CSII-U6-gRNA-CBh-3xFLAG-PA-dCas9-P2A-Puro
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gccgcctctc tcggccttgg tcagattgtc gaactttctc tgggtaatca gcttggcgtt
       61 cagcagctgc cgccagtagt tcttcatctt cttcacgacc tcttcggagg gcatgttgtc
      121 gctcttgccc cggttcttgt cgcttctggt cagcaccttg ttgtcgatgg agtcgtcctt
      181 cagaaagctc tgaggcacga tagcgtccac atcgtagtcg gacagccggt tgatgtccag
      241 ttcctggtcc acgtacatat cccgcccatt ctgcaggtag tacaggtaca gcttctcgtt
      301 ctgcagctgg gtgttttcca cggggtgttc tttcaggatc tggctgccca gctctttgat
      361 gccctcttcg atccgcttca ttctctcgcg gctgttcttc tgtcccttct gggtggtctg
      421 gttctctctg gccatttcga tcacgatgtt ctcgggcttg tgccggccca tcactttcac
      481 gagctcgtcc accaccttca ctgtctgcag gatgcccttc ttaatggcgg ggctgccggc
      541 cagattggca atgtgctcgt gcaggctatc gccctggccg gacacctggg ctttctggat
      601 gtcctcttta aaggtcaggc tgtcgtcgtg gatcagctgc atgaagtttc tgttggcgaa
      661 gccgtcggac ttcaggaaat ccaggattgt cttgccggac tgcttgtccc ggatgccgtt
      721 gatcagcttc cggctcagcc tgccccagcc ggtgtatctc cgccgcttca gctgcttcat
      781 cactt
//
