LOCUS       sequence-164875-        4236 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing FYN , part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4236)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_FYN_WT
FEATURES             Location/Qualifiers
     source          1..4236
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1636..1735)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1752..1771)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1753..1771)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1776..1792)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1776..1792)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2223..3014
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3107..3695
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3596..3615
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3849..3866
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4025..4052)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4144..4230)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcat gggctgtgtg caatgtaagg ataaagaagc aacaaaactg acggaggaga
      241 gggacggcag cctgaaccag agctctgggt accgctatgg cacagacccc acccctcagc
      301 actaccccag cttcggtgtg acctccatcc ccaactacaa caacttccac gcagccgggg
      361 gccaaggact caccgtcttt ggaggtgtga actcttcgtc tcatacgggg accttgcgta
      421 cgagaggagg aacaggagtg acactctttg tggcccttta tgactatgaa gcacggacag
      481 aagatgacct gagttttcac aaaggagaaa aatttcaaat attgaacagc tcggaaggag
      541 attggtggga agcccgctcc ttgacaactg gagagacagg ttacattccc agcaattatg
      601 tggctccagt tgactctatc caggcagaag agtggtactt tggaaaactt ggccgaaaag
      661 atgctgagcg acagctattg tcctttggaa acccaagagg tacctttctt atccgcgaga
      721 gtgaaaccac caaaggtgcc tattcacttt ctatccgtga ttgggatgat atgaaaggag
      781 accatgtcaa acattataaa attcgcaaac ttgacaatgg tggatactac attaccaccc
      841 gggcccagtt tgaaacactt cagcagcttg tacaacatta ctcaggtacc tggaatggaa
      901 acacaaaagt agccataaag actcttaaac caggcacaat gtcccccgaa tcattccttg
      961 aggaagcgca gatcatgaag aagctgaagc acgacaagct ggtccagctc tatgcagtgg
     1021 tgtctgagga gcccatctac atcgtcaccg agtatatgaa caaaggaagt ttactggatt
     1081 tcttaaaaga tggagaagga agagctctga aattaccaaa tcttgtggac atggcagcac
     1141 aggtggctgc aggaatggct tacatcgagc gcatgaatta tatccataga gatctgcgat
     1201 cagcaaacat tctagtgggg aatggactca tatgcaagat tgctgacttc ggattggccc
     1261 gattgataga agacaatgag tacacagcaa gacaaggtgc aaagttcccc atcaagtgga
     1321 cggcccccga ggcagccctg tacgggaggt tcacaatcaa gtctgacgtg tggtcttttg
     1381 gaatcttact cacagagctg gtcaccaaag gaagagtgcc atacccaggc atgaacaacc
     1441 gggaggtgct ggagcaggtg gagcgaggct acaggatgcc ctgcccgcag gactgcccca
     1501 tctctctgca tgagctcatg atccactgct ggaaaaagga ccctgaagaa cgccccactt
     1561 ttgagtactt gcagagcttc ctggaagact actttaccgc gacagagccc cagtaccaac
     1621 ctggtgaaaa cctgtaccca actttcttgt acaaagttgg cattataaga aagcattgct
     1681 tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc
     1741 cagctgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct
     1801 ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgcc
     1861 tcctctagac cagccaggac agaaatgcct cgacttcgct gctacccaag gttgccgggt
     1921 gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc
     1981 gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac
     2041 gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg
     2101 tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga
     2161 tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca
     2221 ttatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca
     2281 tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg
     2341 gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg
     2401 aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga
     2461 gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc
     2521 gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag
     2581 gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag
     2641 aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac
     2701 aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg
     2761 ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg
     2821 gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt
     2881 atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg
     2941 cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg
     3001 tagtcggcaa ataaccctcg agccacccat gaccaaaatc ccttaacgtg agttacgcgt
     3061 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     3121 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     3181 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     3241 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     3301 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     3361 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     3421 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     3481 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     3541 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     3601 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     3661 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     3721 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     3781 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     3841 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     3901 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     3961 cgggcagtga gcgcaacgca attaatacgc gtaccgctag ccaggaagag tttgtagaaa
     4021 cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg
     4081 cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg
     4141 cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca
     4201 gtcttccgac tgagcctttc gttttatttg atgcct
//
