LOCUS       sequence-16488-e         598 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Nathan DF, Vos MH, Lindquist S
  TITLE     In vivo functions of the Saccharomyces cerevisiae Hsp90 chaperone.
  JOURNAL   Proc Natl Acad Sci U S A 1997 Nov 25;94(24):12949-56.
  PUBMED    9371781
REFERENCE   2  (bases 1 to 598)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A"; date: "1997-11-25"; volume: "94(24)"; pages: 
            "12949-56"
COMMENT     Sequence Label: Gal ts-lux
FEATURES             Location/Qualifiers
     source          1..598
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     9..25
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     10..26
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(57..387)
                     /gene="S. cerevisiae GAL10"
                     /label=GAL10 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    270..387
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
ORIGIN
        1 gccccccctc gaggtcgacg gtatcgataa gcttgatatc gaattccttg aattttcaaa
       61 aattcttact ttttttttgg atggacgcaa agaagtttaa taatcatatt acatggcatt
      121 accaccatat acatatccat atacatatcc atatctaatc ttacttatat gttgtggaaa
      181 tgtaaagagc cccattatct tagcctaaaa aaaccttctc tttggaactt tcagtaatac
      241 gcttaactgc tcattgctat attgaagtac ggattaaaag ccgccgagcg ggtgacagcc
      301 ctccgaagga agactctcct ccgtgcgtcc tcgtcttcac cggtcgcgtt cctgaaacgc
      361 agatgtgcct cgcgccgcac tgctccgaac aataaagatt ctacaatact aacttttatg
      421 gttatgaaga agaaaaattg gcagtaacct ggccccacaa accttcaaat gaacgaatca
      481 aattaacaac cataagatga taatgcgatt agttttttaa ccttatttct ggggtaatta
      541 atcaacgaag ccattatttt tgatctaatt acagatatat aaatgcaaaa actgcata
//
