LOCUS       sequence-164888-        4236 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing HDAC1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4236)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_HDAC1_WT
FEATURES             Location/Qualifiers
     source          1..4236
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1636..1735)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1752..1771)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1753..1771)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1776..1792)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1776..1792)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2223..3014
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3107..3695
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3596..3615
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3849..3866
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4025..4052)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4144..4230)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcat ggcgcagacg cagggcaccc ggaggaaagt ctgttactac tacgacgggg
      241 atgttggaaa ttactattat ggacaaggcc acccaatgaa gcctcaccga atccgcatga
      301 ctcataattt gctgctcaac tatggtctct accgaaaaat ggaaatctat cgccctcaca
      361 aagccaatgc tgaggagatg accaagtacc acagcgatga ctacattaaa ttcttgcgct
      421 ccatccgtcc agataacatg tcggagtaca gcaagcagat gcagagattc aacgttggtg
      481 aggactgtcc agtattcgat ggcctgtttg agttctgtca gttgtctact ggtggttctg
      541 tggcaagtgc tgtgaaactt aataagcagc agacggacat cgctgtgaat tgggctgggg
      601 gcctgcacca tgcaaagaag tccgaggcat ctggcttctg ttacgtcaat gatatcgtct
      661 tggccatcct ggaactgcta aagtatcacc agagggtgct gtacattgac attgatattc
      721 accatggtga cggcgtggaa gaggccttct acaccacgga ccgggtcatg actgtgtcct
      781 ttcataagta tggagagtac ttcccaggaa ctggggacct acgggatatc ggggctggca
      841 aaggcaagta ttatgctgtt aactacccgc tccgagacgg gattgatgac gagtcctatg
      901 aggccatttt caagccggtc atgtccaaag taatggagat gttccagcct agtgcggtgg
      961 tcttacagtg tggctcagac tccctatctg gggatcggtt aggttgcttc aatctaacta
     1021 tcaaaggaca cgccaagtgt gtggaatttg tcaagagctt taacctgcct atgctgatgc
     1081 tgggaggcgg tggttacacc attcgtaacg ttgcccggtg ctggacatat gagacagctg
     1141 tggccctgga tacggagatc cctaatgagc ttccatacaa tgactacttt gaatactttg
     1201 gaccagattt caagctccac atcagtcctt ccaatatgac taaccagaac acgaatgagt
     1261 acctggagaa gatcaaacag cgactgtttg agaaccttag aatgctgccg cacgcacctg
     1321 gggtccaaat gcaggcgatt cctgaggacg ccatccctga ggagagtggc gatgaggacg
     1381 aagacgaccc tgacaagcgc atctcgatct gctcctctga caaacgaatt gcctgtgagg
     1441 aagagttctc cgattctgaa gaggagggag aggggggccg caagaactct tccaacttca
     1501 aaaaagccaa gagagtcaaa acagaggatg aaaaagagaa agacccagag gagaagaaag
     1561 aagtcaccga agaggagaaa accaaggagg agaagccaga agccaaaggg gtcaaggagg
     1621 aggtcaagtt ggcctgccca actttcttgt acaaagttgg cattataaga aagcattgct
     1681 tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc
     1741 cagctgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct
     1801 ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgcc
     1861 tcctctagac cagccaggac agaaatgcct cgacttcgct gctacccaag gttgccgggt
     1921 gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc
     1981 gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac
     2041 gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg
     2101 tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga
     2161 tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca
     2221 ttatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca
     2281 tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg
     2341 gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg
     2401 aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga
     2461 gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc
     2521 gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag
     2581 gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag
     2641 aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac
     2701 aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg
     2761 ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg
     2821 gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt
     2881 atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg
     2941 cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg
     3001 tagtcggcaa ataaccctcg agccacccat gaccaaaatc ccttaacgtg agttacgcgt
     3061 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     3121 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     3181 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     3241 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     3301 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     3361 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     3421 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     3481 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     3541 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     3601 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     3661 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     3721 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     3781 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     3841 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     3901 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     3961 cgggcagtga gcgcaacgca attaatacgc gtaccgctag ccaggaagag tttgtagaaa
     4021 cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg
     4081 cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg
     4141 cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca
     4201 gtcttccgac tgagcctttc gttttatttg atgcct
//
