LOCUS       sequence-16489-e         514 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Nathan DF, Vos MH, Lindquist S
  TITLE     In vivo functions of the Saccharomyces cerevisiae Hsp90 chaperone.
  JOURNAL   Proc Natl Acad Sci U S A 1997 Nov 25;94(24):12949-56.
  PUBMED    9371781
REFERENCE   2  (bases 1 to 514)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A"; date: "1997-11-25"; volume: "94(24)"; pages: 
            "12949-56"
COMMENT     Sequence Label: Gal tr-lux
FEATURES             Location/Qualifiers
     source          1..514
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     29..45
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     30..46
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(77..407)
                     /gene="S. cerevisiae GAL10"
                     /label=GAL10 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    290..407
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
ORIGIN
        1 gaacaaaagc ttggtaccgg gccccccctc gaggtcgacg gtatcgataa gcttgatatc
       61 gaattccttg aattttcaaa aattcttact ttttttttgg atggacgcaa agaagtttaa
      121 taatcatatt acatggcatt accaccatat acatatccat atacatatcc atatctaatc
      181 ttacttatat gttgtggaaa tgtaaagagc cccattatct tagcctaaaa aaaccttctc
      241 tttggaactt tcagtaatac gcttaactgc tcattgctat attgaagtac ggattagaag
      301 ccgccgagcg ggtgacagcc ctccgaagga agactctcct ccgtgcgtcc tcgtcttcac
      361 cggtcgcgtt cctgaaacgc agatgtgcct cgcgccgcac tgctccgaac aataaagatt
      421 ctacaatact agcttttatg gttatgaaga ggaaaaattg gcagtaacct ggccccacga
      481 accttcaaat gaacgaatca aattaacaac cata
//
