LOCUS       sequence-164897-        4329 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing LYN ,part of the Target Accelerator 
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4329)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4329)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_LYN_WT
FEATURES             Location/Qualifiers
     source          1..4329
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1729..1828)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1845..1864)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1846..1864)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1869..1885)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1869..1885)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2316..3107
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3200..3788
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3689..3708
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3942..3959
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4118..4145)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4237..4323)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catgggatgt ataaaatcaa aagggaaaga cagcttgagt gacgatggag
      241 tagatttgaa gactcaacca gtacgtaata ctgaaagaac tatttatgtg agagatccaa
      301 cgtccaataa acagcaaagg ccagttccag aatctcagct tttacctgga cagaggtttc
      361 aaactaaaga tccagaggaa caaggagaca ttgtggtagc cttgtacccc tatgatggca
      421 tccacccgga cgacttgtct ttcaagaaag gagagaagat gaaagtcctg gaggagcatg
      481 gagaatggtg gaaagcaaag tcccttttaa caaaaaaaga aggcttcatc cccagcaact
      541 atgtggccaa actcaacacc ttagaaacag aagagtggtt tttcaaggat ataaccagga
      601 aggacgcaga aaggcagctt ttggcaccag gaaatagcgc tggagctttc cttattagag
      661 aaagtgaaac attaaaagga agcttctctc tgtctgtcag agactttgac cctgtgcatg
      721 gtgatgttat taagcactac aaaattagaa gtctggataa tgggggctat tacatctctc
      781 cacgaatcac ttttccctgt atcagcgaca tgattaaaca ttaccaaaag caggcagatg
      841 gcttgtgcag aagattggag aaggcttgta ttagtcccaa gccacagaag ccatgggata
      901 aagatgcctg ggagatcccc cgggagtcca tcaagttggt gaaaaggctt ggcgctgggc
      961 agtttgggga agtctggatg ggttactata acaacagtac caaggtggct gtgaaaaccc
     1021 tgaagccagg aactatgtct gtgcaagcct tcctggaaga agccaacctc atgaagaccc
     1081 tgcagcatga caagctcgtg aggctctacg ctgtggtcac cagggaggag cccatttaca
     1141 tcatcaccga gtacatggcc aagggcagtt tgctggattt cctgaagagc gatgaaggtg
     1201 gcaaagtgct gcttccaaag ctcattgact tttctgctca gattgcagag ggaatggcat
     1261 acatcgagcg gaagaactac attcaccggg acctgcgagc agctaatgtt ctggtctccg
     1321 agtcactcat gtgcaaaatt gcagattttg gccttgctag agtaattgaa gataatgagt
     1381 acacagcaag ggaaggtgct aagttcccta ttaagtggac ggctccagaa gcaatcaact
     1441 ttggatgttt cactattaag tctgatgtgt ggtcctttgg aatcctccta tacgaaattg
     1501 tcacctatgg gaaaattccc tacccaggga gaactaatgc cgacgtgatg accgccctgt
     1561 cccagggcta caggatgccc cgtgtggaga actgcccaga tgagctctat gacattatga
     1621 aaatgtgctg gaaagaaaag gcagaagaga gaccaacgtt tgactactta cagagcgtcc
     1681 tggatgattt ctacacagcc acggaagggc aataccagca gcagcctttg ccaactttct
     1741 tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca
     1801 ctatcagtca aaataaaatc attatttgcc atccagctga tatcccctat agtgagtcgt
     1861 attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt
     1921 acattgcaca agataaaaat atatcatcat gcctcctcta gaccagccag gacagaaatg
     1981 cctcgacttc gctgctaccc aaggttgccg ggtgacgcac accgtggaaa cggatgaagg
     2041 cacgaaccca gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc
     2101 gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg
     2161 ttttcatggc ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc
     2221 agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc
     2281 agcagggcag tcgccctaaa acaaagttaa acattatgag ggaagcggtg atcgccgaag
     2341 tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc
     2401 tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg
     2461 atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg
     2521 accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca
     2581 ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat
     2641 ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca
     2701 ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag
     2761 cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa
     2821 ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta
     2881 cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg
     2941 ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac
     3001 aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat
     3061 ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg caaataaccc tcgagccacc
     3121 catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag
     3181 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     3241 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     3301 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     3361 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     3421 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     3481 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     3541 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     3601 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     3661 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     3721 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     3781 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     3841 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     3901 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     3961 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     4021 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata
     4081 cgcgtaccgc tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc
     4141 cttctgctta gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg
     4201 ccgttgcttc acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt
     4261 caccgacaaa caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat
     4321 ttgatgcct
//
