LOCUS       sequence-16490-e         709 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Meriin AB, Zhang X, He X, Newnam GP, Chernoff YO, Sherman MY
  TITLE     Huntington toxicity in yeast model depends on polyglutamine 
            aggregation mediated by a prion-like protein Rnq1.
  JOURNAL   J Cell Biol 2002 Jun 10;157(6):997-1004. Epub 2002 Jun 10.
  PUBMED    12058016
REFERENCE   2  (bases 1 to 709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol"; date: "2002-06-10"; volume: "157(6)"; pages: "997-1004. Epub 
            2002 Jun 10"
COMMENT     Sequence Label: pYES2/103Q
FEATURES             Location/Qualifiers
     source          1..709
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..32
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        13..31
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             57..80
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(486..507)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tactagcagc tgtaatacga ctcactatag ggaatattaa gcttggtacc gccatggact
       61 acaaggacga cgatgacaag ctggcgaccc tggaaaagct gatgaaggcc ttcgagtccc
      121 tcaaaagctt ccaacagcag caacagcaac aacagcagca acagcaacaa cagcagcaac
      181 agcaacaaca gcagcaacag caacaacagc agcaacagca acaacagcag caacagcaac
      241 aacagcagca acagcaacaa cagcagcaac agcaacaaca gcagcaacag caacaacagc
      301 agcaacagca acaacagcag caacagcaac aacagcagca acagcaacaa cagcagcaac
      361 agcaacaaca gcagcaacag caacaacagc agcaacagca acaacagcag caacagcaac
      421 aactgcagcc cgggggatcc ctggtgagca agggcgagga gctgttcacc ggggtggtgc
      481 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
      541 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
      601 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
      661 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgcc
//
