LOCUS       sequence-164904-        1041 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  csd4, construct: 22-438, pET28b(+) N-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Kim HS, Kim J, Im HN, An DR, Lee M, Hesek D, Mobashery S, Kim JY, 
            Cho K, Yoon HJ, Han BW, Lee BI, Suh SW
  TITLE     Structural basis for the recognition of muramyltripeptide by 
            Helicobacter pylori Csd4, a D,L-carboxypeptidase controlling the 
            helical cell shape.
  JOURNAL   Acta Crystallogr D Biol Crystallogr. 2014 Nov;70(Pt 11):2800-12. 
            doi: 10.1107/S1399004714018732. Epub 2014 Oct 16.
  PUBMED    25372672
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Acta 
            Crystallogr D Biol Crystallogr."; date: "2014-11"; volume: "70(Pt 
            11):2800-12. doi"; pages: " 10.1107/S1399004714018732. Epub 2014 Oct
            16"
COMMENT     Sequence Label: csd4_22-438/pET28b(+)
FEATURES             Location/Qualifiers
     source          1..1041
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             57..74
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             84..101
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ccctctagaa ataattttgt ttaactttaa gaaggagata taccatgggc agcagccatc
       61 atcatcatca tcacagcagc ggcctggtgc cgcgcggcag ccatatgata gaaaaagccc
      121 ctacaaatgt agaggataga gacaaagccc cccatttgtt gcttttagca gggattcaag
      181 gcgatgagcc tggtgggttt aatgcaacta atttgttttt aatgcattat agcgttttaa
      241 aaggtttggt tgaagtggtt cctgtattga ataagccttc catgttaaga aatcataggg
      301 gcttgtatgg ggatatgaac cgcaaatttg ccgctttaga caagaatgac cctgaatacc
      361 ccactatcca ggaaatcaaa tccttgattg caaaacccag tatagacgct gtcttgcatt
      421 tgcatgatgg cggtgggtat taccgccctg tttatgttga tgcgatgctc aatcctaagc
      481 gctgggggaa ttgctttatt attgatcaag atgaggttaa aggggcgaaa ttccctaatt
      541 tgcttgcttt tgcaaacaat acgattgaga gtatcaacgc ccatttattg caccccattg
      601 aagagtatca tttaaaaaac acgcgcaccg cgcaaggcga tacagaaatg caaaaagccc
      661 taacttttta tgcgatcaac caaaaaaaga gcgcttttgc caatgaagct agcaaagaac
      721 tccctttagc atcaagggtg ttttaccacc tgcaagccat tgagggctta ctcaatcagc
      781 tcaatatccc ttttaagcgc gattttgatc ttaaccctaa cagcgtgcat gccctaatca
      841 atgataaaaa cttgtgggca aaaatcagct ctttgcctaa aatgcccctt tttaacttgc
      901 gccctaaact caatcatttc cccttacccc acaacactaa aatcccacaa atccccatag
      961 agagcaacgc ttacattgta gggctagtca aaaataaaca agaagtgttt ttaaaatacg
     1021 gcaacaagct catgacacga t
//
