LOCUS       sequence-164906-        3828 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing MOS , part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_MOS_WT
FEATURES             Location/Qualifiers
     source          1..3828
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             144..935
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1028..1616
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1517..1536
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1770..1787
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1946..1973)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2065..2151)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2200..2222
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2214..2231
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2215..2231
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2247..2346
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3385..3484)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3501..3520)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3502..3520)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3525..3541)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3525..3541)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt
       61 acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc
      121 gccctaaaac aaagttaaac attatgaggg aagcggtgat cgccgaagta tcgactcaac
      181 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
      241 tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta
      301 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
      361 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
      421 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
      481 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
      541 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
      601 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
      661 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
      721 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
      781 tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg
      841 gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg
      901 tgaaaggcga gatcaccaag gtagtcggca aataaccctc gagccaccca tgaccaaaat
      961 cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     1021 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     1081 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     1141 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     1201 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     1261 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     1321 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     1381 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     1441 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     1501 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     1561 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     1621 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     1681 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     1741 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     1801 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     1861 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta
     1921 gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttagt
     1981 ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac
     2041 aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca
     2101 acagataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattt gatgcctggc
     2161 agttccctac tctcgcgtta acgctagcat ggatgttttc ccagtcacga cgttgtaaaa
     2221 cgacggccag tcttaagctc gggccccaaa taatgatttt attttgactg atagtgacct
     2281 gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag
     2341 ttggcatgcc ctcgcccctg gccctacgcc cctacctccg gagcgagttt tccccatcgg
     2401 tggacgcgcg gccctgcagc agtccctcag agctacctgc gaagctgctt ctgggggcca
     2461 ctcttcctcg ggccccgcgg ctgccgcgcc ggctggcctg gtgctccatt gactgggagc
     2521 aggtgtgctt gctgcagagg ctgggagctg gagggtttgg ctcggtgtac aaggcgactt
     2581 accgcggtgt tcctgtggcc ataaagcaag tgaacaagtg caccaagaac cgactagcat
     2641 ctcggcggag tttctgggct gagctcaacg tagcaaggct gcgccacgat aacatcgtgc
     2701 gcgtggtggc tgccagcacg cgcacgcccg cagggtccaa tagcctaggg accatcatca
     2761 tggagttcgg tggcaacgtc actttacacc aagtcatcta tggcgccgcc ggccaccctg
     2821 agggggacgc aggggagcct cactgccgca ctggaggaca gttaagtttg ggaaagtgtc
     2881 tcaagtactc actagatgtt gtgaacggcc tgctcttcct ccactcgcaa agcattgtgc
     2941 acttggacct gaagcccgcg aacatcttga tcagtgagca ggatgtctgt aaaattagtg
     3001 acttcggttg ctctgagaag ttggaagatc tgctgtgctt ccagacaccc tcttaccctc
     3061 taggaggcac atacacccac cgcgccccgg agctcctgaa aggagagggc gtgacgccta
     3121 aagccgacat ttattccttt gccatcactc tctggcaaat gactaccaag caggcgccgt
     3181 attcggggga gcggcagcac atactgtacg cggtggtggc ctacgacctg cgcccgtccc
     3241 tctccgctgc cgtcttcgag gactcgctcc ccgggcagcg ccttggggac gtcatccagc
     3301 gctgctggag acccagcgcg gcgcagaggc cgagcgcgcg gctgcttttg gtggatctca
     3361 cctctttgaa agctgaactc ggctgcccaa ctttcttgta caaagttggc attataagaa
     3421 agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta
     3481 tttgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct
     3541 ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat
     3601 catcatgcct cctctagacc agccaggaca gaaatgcctc gacttcgctg ctacccaagg
     3661 ttgccgggtg acgcacaccg tggaaacgga tgaaggcacg aacccagtgg acataagcct
     3721 gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct
     3781 tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggctt
//
