LOCUS       sequence-164913-        6003 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing PIK3CB , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6003)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 6003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_PIK3CB_WT
FEATURES             Location/Qualifiers
     source          1..6003
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             400..408
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(1707..1718)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(3403..3502)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3519..3538)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3520..3538)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3543..3559)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3543..3559)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3990..4781
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4874..5462
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5363..5382
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5616..5633
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5792..5819)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5911..5997)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catgtgcttc agtttcataa tgcctcctgc tatggcagac atccttgaca
      241 tctgggcggt ggattcacag atagcatctg atggctccat acctgtggat ttccttttgc
      301 ccactgggat ttatatccag ttggaggtac ctcgggaagc taccatttct tatattaagc
      361 agatgttatg gaagcaagtt cacaattacc caatgttcaa cctccttatg gatattgact
      421 cctatatgtt tgcatgtgtg aatcagactg ctgtatatga ggagcttgaa gatgaaacac
      481 gaagactctg tgatgtcaga ccttttcttc cagttctcaa attagtgaca agaagttgtg
      541 acccagggga aaaattagac tcaaaaattg gagtccttat aggaaaaggt ctgcatgaat
      601 ttgattcctt gaaggatcct gaagtaaatg aatttcgaag aaaaatgcgc aaattcagcg
      661 aggaaaaaat cctgtcactt gtgggattgt cttggatgga ctggctaaaa caaacatatc
      721 caccagagca tgaaccatcc atccctgaaa acttagaaga taaactttat gggggaaagc
      781 tcatcgtagc tgttcatttt gaaaactgcc aggacgtgtt tagctttcaa gtgtctccta
      841 atatgaatcc tatcaaagta aatgaattgg caatccaaaa acgtttgact attcatggga
      901 aggaagatga agttagcccc tatgattatg tgttgcaagt cagcgggaga gtagaatatg
      961 tttttggtga tcatccacta attcagttcc agtatatccg gaactgtgtg atgaacagag
     1021 ccctgcccca ttttatactt gtggaatgct gcaagatcaa gaaaatgtat gaacaagaaa
     1081 tgattgccat agaggctgcc ataaatcgaa attcatctaa tcttcctctt ccattaccac
     1141 caaagaaaac acgaattatt tctcatgttt gggaaaataa caaccctttc caaattgtct
     1201 tggttaaggg aaataaactt aacacagagg aaactgtaaa agttcatgtc agggctggtc
     1261 tttttcatgg tactgagctc ctgtgtaaaa ccatcgtaag ctcagaggta tcagggaaaa
     1321 atgatcatat ttggaatgaa ccactggaat ttgatattaa tatttgtgac ttaccaagaa
     1381 tggctcgatt atgttttgct gtttatgcag ttttggataa agtaaaaacg aagaaatcaa
     1441 cgaaaactat taatccctct aaatatcaga ccatcaggaa agctggaaaa gtgcattatc
     1501 ctgtagcgtg ggtaaatacg atggtttttg actttaaagg acaattgaga actggagaca
     1561 taatattaca cagctggtct tcatttcctg atgaactcga agaaatgttg aatccaatgg
     1621 gaactgttca aacaaatcca tatactgaaa atgcaacagc tttgcatgtt aaatttccag
     1681 agaataaaaa acaaccttat tattaccctc ccttcgataa gattattgaa aaggcagctg
     1741 agattgcaag cagtgatagt gctaatgtgt caagtcgagg tggaaaaaag tttcttcctg
     1801 tattgaaaga aatcttggac agggatccct tgtctcaact gtgtgaaaat gaaatggatc
     1861 ttatttggac tttgcgacaa gactgccgag agattttccc acaatcactg ccaaaattac
     1921 tgctgtcaat caagtggaat aaacttgagg atgttgctca gcttcaggcg ctgcttcaga
     1981 tttggcctaa actgcccccc cgggaggccc tagagcttct ggatttcaac tatccagacc
     2041 agtacgttcg agaatatgct gtaggctgcc tgcgacagat gagtgatgaa gaactttctc
     2101 aatatctttt acaactggtg caagtgttaa aatatgagcc ttttcttgat tgtgccctct
     2161 ctagattcct attagaaaga gcacttggta atcggaggat agggcagttt ctattttggc
     2221 atcttaggtc agaagtgcac attcctgctg tctcagtaca atttggtgtc atccttgaag
     2281 catactgccg gggaagtgtg gggcacatga aagtgctttc taagcaggtt gaagcactca
     2341 ataagttaaa aactttaaat agtttaatca aactgaatgc cgtgaagtta aacagagcca
     2401 aagggaagga ggccatgcat acctgtttaa aacagagtgc ttaccgggaa gccctctctg
     2461 acctgcagtc acccctgaac ccatgtgtta tcctctcaga actctatgtt gaaaagtgca
     2521 aatacatgga ttccaaaatg aagcctttgt ggctggtata caataacaag gtatttggtg
     2581 aggattcagt tggagtgatt tttaaaaatg gtgatgattt acgacaggat atgttgacac
     2641 tccaaatgtt gcgcttgatg gatttactct ggaaagaagc tggtttggat cttcggatgt
     2701 tgccttatgg ctgtttagca acaggagatc gctctggcct cattgaagtt gtgagcacct
     2761 ctgaaacaat tgctgacatt cagctgaaca gtagcaatgt ggctgctgca gcagccttca
     2821 acaaagatgc ccttctgaac tggcttaaag aatacaactc tggggatgac ctggaccgag
     2881 ccattgagga atttacactg tcctgtgctg gctactgtgt agcttcttat gtccttggga
     2941 ttggtgacag acatagtgac aacatcatgg tcaaaaaaac tggccagctc ttccacattg
     3001 actttggaca tattcttgga aatttcaaat ctaagtttgg cattaaaagg gagcgagtgc
     3061 cttttattct tacctatgat ttcatccatg tcattcaaca aggaaaaaca ggaaatacag
     3121 aaaagtttgg ccggttccgc cagtgttgtg aggatgcata tctgatttta cgacggcatg
     3181 ggaatctctt catcactctc tttgcgctga tgttgactgc agggcttcct gaactcacat
     3241 cagtcaaaga tatacagtat cttaaggact ctcttgcatt agggaagagt gaagaagaag
     3301 cactcaaaca gtttaagcaa aaatttgatg aggcgctcag ggaaagctgg actactaaag
     3361 tgaactggat ggcccacaca gttcggaaag actacagatc tttgccaact ttcttgtaca
     3421 aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca
     3481 gtcaaaataa aatcattatt tgccatccag ctgatatccc ctatagtgag tcgtattaca
     3541 tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg
     3601 cacaagataa aaatatatca tcatgcctcc tctagaccag ccaggacaga aatgcctcga
     3661 cttcgctgct acccaaggtt gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa
     3721 cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac
     3781 gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca
     3841 tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag
     3901 cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg
     3961 gcagtcgccc taaaacaaag ttaaacatta tgagggaagc ggtgatcgcc gaagtatcga
     4021 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
     4081 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc
     4141 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt
     4201 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg
     4261 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag
     4321 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc
     4381 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg
     4441 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa
     4501 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt
     4561 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact
     4621 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt
     4681 atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc
     4741 actacgtgaa aggcgagatc accaaggtag tcggcaaata accctcgagc cacccatgac
     4801 caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga
     4861 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     4921 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     4981 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     5041 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     5101 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     5161 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     5221 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     5281 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     5341 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     5401 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     5461 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     5521 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     5581 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     5641 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     5701 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta
     5761 ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga tggccttctg
     5821 cttagtttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg
     5881 cttcacaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag cgttcaccga
     5941 caaacaacag ataaaacgaa aggcccagtc ttccgactga gcctttcgtt ttatttgatg
     6001 cct
//
