LOCUS       sequence-164914-        1052 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  csd5, construct: 41-192, pET28b(+) N-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Kim HS, Kim J, Im HN, An DR, Lee M, Hesek D, Mobashery S, Kim JY, 
            Cho K, Yoon HJ, Han BW, Lee BI, Suh SW
  TITLE     Structural basis for the recognition of muramyltripeptide by 
            Helicobacter pylori Csd4, a D,L-carboxypeptidase controlling the 
            helical cell shape.
  JOURNAL   Acta Crystallogr D Biol Crystallogr. 2014 Nov;70(Pt 11):2800-12. 
            doi: 10.1107/S1399004714018732. Epub 2014 Oct 16.
  PUBMED    25372672
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Acta 
            Crystallogr D Biol Crystallogr."; date: "2014-11"; volume: "70(Pt 
            11):2800-12. doi"; pages: " 10.1107/S1399004714018732. Epub 2014 Oct
            16"
COMMENT     Sequence Label: csd5_41-192/pET28b(+)
FEATURES             Location/Qualifiers
     source          1..1052
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             57..74
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             84..101
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             573..590
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(643..661)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      657..704
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(828..847)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ccctctagaa ataattttgt ttaactttaa gaaggagata taccatgggc agcagccatc
       61 atcatcatca tcacagcagc ggcctggtgc cgcgcggcag ccatatgagc gctccaataa
      121 gcccaaatgt agaaaaaacc gagacagagc gtcaaaacgg cgtgctttcg cccaaacaag
      181 aagaagccaa cgcaaccaca actgccacag aagaaagccc caccaaagac acagcgccgc
      241 ctttagacac agccgcgcaa aaacaagaaa ctaaacaaga gcaagaaaaa gaaaacgagc
      301 ctaaacaaga tagcgtcccg cccgttcaaa acaatcaaaa aacccctaca acccccttaa
      361 tgggaaaaaa acctttagag tataaagtcg cagtcagtgg cgtgaatgtg cgcgcttttc
      421 ccagcacaaa aggtaaaatc ttgggattgc ttttaaaaaa taaaagcgtg aaagttttag
      481 aaatccaaaa cgattgggct gaaattgaat tttctcacga aacaaagggc tatgtgtttt
      541 taaaactttt aaaaaaggct gaataactcg agcaccacca ccaccaccac tgagatccgg
      601 ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag
      661 cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta
      721 tatccggatt ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
      781 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
      841 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
      901 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
      961 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     1021 gtccacgttc tttaatagtg gactcttgtt cc
//
