LOCUS       sequence-164915-        1014 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  csd5, construct: 41-192, pET28b(+) N-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Kim HS, Kim J, Im HN, An DR, Lee M, Hesek D, Mobashery S, Kim JY, 
            Cho K, Yoon HJ, Han BW, Lee BI, Suh SW
  TITLE     Structural basis for the recognition of muramyltripeptide by 
            Helicobacter pylori Csd4, a D,L-carboxypeptidase controlling the 
            helical cell shape.
  JOURNAL   Acta Crystallogr D Biol Crystallogr. 2014 Nov;70(Pt 11):2800-12. 
            doi: 10.1107/S1399004714018732. Epub 2014 Oct 16.
  PUBMED    25372672
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Acta 
            Crystallogr D Biol Crystallogr."; date: "2014-11"; volume: "70(Pt 
            11):2800-12. doi"; pages: " 10.1107/S1399004714018732. Epub 2014 Oct
            16"
COMMENT     Sequence Label: csd5_41-192/pET28b(+)
FEATURES             Location/Qualifiers
     source          1..1014
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(524..541)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(551..568)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             587..609
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    624..648
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(648..667)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(649..667)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(751..770)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtt
       61 attcagcctt ttttaaaagt tttaaaaaca catagccctt tgtttcgtga gaaaattcaa
      121 tttcagccca atcgttttgg atttctaaaa ctttcacgct tttatttttt aaaagcaatc
      181 ccaagatttt accttttgtg ctgggaaaag cgcgcacatt cacgccactg actgcgactt
      241 tatactctaa aggttttttt cccattaagg gggttgtagg ggttttttga ttgttttgaa
      301 cgggcgggac gctatcttgt ttaggctcgt tttctttttc ttgctcttgt ttagtttctt
      361 gtttttgcgc ggctgtgtct aaaggcggcg ctgtgtcttt ggtggggctt tcttctgtgg
      421 cagttgtggt tgcgttggct tcttcttgtt tgggcgaaag cacgccgttt tgacgctctg
      481 tctcggtttt ttctacattt gggcttattg gagcgctcat atggctgccg cgcggcacca
      541 ggccgctgct gtgatgatga tgatgatggc tgctgcccat ggtatatctc cttcttaaag
      601 ttaaacaaaa ttatttctag aggggaattg ttatccgctc acaattcccc tatagtgagt
      661 cgtattaatt tcgcgggatc gagatctcga tcctctacgc cggacgcatc gtggccggca
      721 tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag
      781 atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc
      841 ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg
      901 tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag
      961 agcgtcgaga tcccggacac catcgaatgg cgcaaaacct ttcgcggtat ggca
//
