LOCUS       sequence-164917-        5925 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing PIK3CD , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5925)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 5925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_PIK3CD_WT
FEATURES             Location/Qualifiers
     source          1..5925
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3325..3424)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3441..3460)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3442..3460)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3465..3481)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3465..3481)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3912..4703
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4796..5384
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5285..5304
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5538..5555
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5714..5741)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5833..5919)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catgccccct ggggtggact gccccatgga attctggacc aaggaggaga
      241 atcagagcgt tgtggttgac ttcctgctgc ccacaggggt ctacctgaac ttccctgtgt
      301 cccgcaatgc caacctcagc accatcaagc agctgctgtg gcaccgcgcc cagtatgagc
      361 cgctcttcca catgctcagt ggccccgagg cctatgtgtt cacctgcatc aaccagacag
      421 cggagcagca agagctggag gacgagcaac ggcgtctgtg tgacgtgcag cccttcctgc
      481 ccgtcctgcg cctggtggcc cgtgagggcg accgcgtgaa gaagctcatc aactcacaga
      541 tcagcctcct catcggcaaa ggcctccacg agtttgactc cttgtgcgac ccagaagtga
      601 acgactttcg cgccaagatg tgccaattct gcgaggaggc ggccgcccgc cggcagcagc
      661 tgggctggga ggcctggctg cagtacagtt tccccctgca gctggagccc tcggctcaaa
      721 cctgggggcc tggtaccctg cggctcccga accgggccct tctggtcaac gttaagtttg
      781 agggcagcga ggagagcttc accttccagg tgtccaccaa ggacgtgccg ctggcgctga
      841 tggcctgtgc cctgcggaag aaggccacag tgttccggca gccgctggtg gagcagccgg
      901 aagactacac gctgcaggtg aacggcaggc atgagtacct gtatggcagc tacccgctct
      961 gccagttcca gtacatctgc agctgcctgc acagtgggtt gacccctcac ctgaccatgg
     1021 tccattcctc ctccatcctc gccatgcggg atgagcagag caaccctgcc ccccaggtcc
     1081 agaaaccgcg tgccaaacca cctcccattc ctgcgaagaa gccttcctct gtgtccctgt
     1141 ggtccctgga gcagccgttc cgcatcgagc tcatccaggg cagcaaagtg aacgccgacg
     1201 agcggatgaa gctggtggtg caggccgggc ttttccacgg caacgagatg ctgtgcaaga
     1261 cggtgtccag ctcggaggtg agcgtgtgct cggagcccgt gtggaagcag cggctggagt
     1321 tcgacatcaa catctgcgac ctgccccgca tggcccgtct ctgctttgcg ctgtacgccg
     1381 tgatcgagaa agccaagaag gctcgctcca ccaagaagaa gtccaagaag gcggactgcc
     1441 ccattgcctg ggccaacctc atgctgtttg actacaagga ccagcttaag accggggaac
     1501 gctgcctcta catgtggccc tccgtcccag atgagaaggg cgagctgctg aaccccacgg
     1561 gcactgtgcg cagtaacccc aacacggata gcgccgctgc cctgctcatc tgcctgcccg
     1621 aggtggcccc gcaccccgtg tactaccccg ccctggagaa gatcttggag ctggggcgac
     1681 acagcgagtg tgtgcatgtc accgaggagg agcagctgca gctgcgggaa atcctggagc
     1741 ggcgggggtc tggggagctg tatgagcacg agaaggacct ggtgtggaag ctgcggcatg
     1801 aagtccagga gcacttcccg gaggcgctag cccggctgct gctggtcacc aagtggaaca
     1861 agcatgagga tgtggcccag atgctctacc tgctgtgctc ctggccggag ctgcccgtcc
     1921 tgagcgccct ggagctgcta gacttcagct tccccgattg ccacgtaggc tccttcgcca
     1981 tcaagtcgct gcggaaactg acggacgatg agctgttcca gtacctgctg cagctggtgc
     2041 aggtgctcaa gtacgagtcc tacctggact gcgagctgac caaattcctg ctggaccggg
     2101 ccctggccaa ccgcaagatc ggccacttcc ttttctggca cctccgctcc gagatgcacg
     2161 tgccgtcggt ggccctgcgc ttcggcctca tcctggaggc ctactgcagg ggcagcaccc
     2221 accacatgaa ggtgctgatg aagcaggggg aagcactgag caaactgaag gccctgaatg
     2281 acttcgtcaa gctgagctct cagaagaccc ccaagcccca gaccaaggag ctgatgcact
     2341 tgtgcatgcg gcaggaggcc tacctagagg ccctctccca cctgcagtcc ccactcgacc
     2401 ccagcaccct gctggctgaa gtctgcgtgg agcagtgcac cttcatggac tccaagatga
     2461 agcccctgtg gatcatgtac agcaacgagg aggcaggcag cggcggcagc gtgggcatca
     2521 tctttaagaa cggggatgac ctccggcagg acatgctgac cctgcagatg atccagctca
     2581 tggacgtcct gtggaagcag gaggggctgg acctgaggat gaccccctat ggctgcctcc
     2641 ccaccgggga ccgcacaggc ctcattgagg tggtactccg ttcagacacc atcgccaaca
     2701 tccaactcaa caagagcaac atggcagcca cagccgcctt caacaaggat gccctgctca
     2761 actggctgaa gtccaagaac ccgggggagg ccctggatcg agccattgag gagttcaccc
     2821 tctcctgtgc tggctattgt gtggccacat atgtgctggg cattggcgat cggcacagcg
     2881 acaacatcat gatccgagag agtgggcagc tgttccacat tgattttggc cactttctgg
     2941 ggaatttcaa gaccaagttt ggaatcaacc gcgagcgtgt cccattcatc ctcacctacg
     3001 actttgtcca tgtgattcag caggggaaga ctaataatag tgagaaattt gaacggttcc
     3061 ggggctactg tgaaagggcc tacaccatcc tgcggcgcca cgggcttctc ttcctccacc
     3121 tctttgccct gatgcgggcg gcaggcctgc ctgagctcag ctgctccaaa gacatccagt
     3181 atctcaagga ctccctggca ctggggaaaa cagaggagga ggcactgaag cacttccgag
     3241 tgaagtttaa cgaagccctc cgtgagagct ggaaaaccaa agtgaactgg ctggcccaca
     3301 acgtgtccaa agacaacagg cagttgccaa ctttcttgta caaagttggc attataagaa
     3361 agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta
     3421 tttgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct
     3481 ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat
     3541 catcatgcct cctctagacc agccaggaca gaaatgcctc gacttcgctg ctacccaagg
     3601 ttgccgggtg acgcacaccg tggaaacgga tgaaggcacg aacccagtgg acataagcct
     3661 gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct
     3721 tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt
     3781 tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc
     3841 gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa
     3901 agttaaacat tatgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag
     3961 ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg
     4021 cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa
     4081 ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc
     4141 ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca
     4201 ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca
     4261 ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa
     4321 aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg
     4381 ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc
     4441 ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg
     4501 cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc
     4561 cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag
     4621 atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga
     4681 tcaccaaggt agtcggcaaa taaccctcga gccacccatg accaaaatcc cttaacgtga
     4741 gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     4801 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     4861 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     4921 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     4981 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     5041 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     5101 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     5161 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     5221 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     5281 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     5341 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     5401 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     5461 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     5521 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     5581 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     5641 actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc caggaagagt
     5701 ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt gatgcctggc
     5761 agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc
     5821 cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg
     5881 aaaggcccag tcttccgact gagcctttcg ttttatttga tgcct
//
