LOCUS       sequence-164919-         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent probe for M/G1 transition.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Bajar BT, Lam AJ, Badiee RK, Oh YH, Chu J, Zhou XX, Kim N, Kim BB, 
            Chung M, Yablonovitch AL, Cruz BF, Kulalert K, Tao JJ, Meyer T, Su 
            XD, Lin MZ
  TITLE     Fluorescent indicators for simultaneous reporting of all four cell 
            cycle phases.
  JOURNAL   Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4045.
  PUBMED    27798610
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Oct 31. doi: 10.1038/nmeth.4045."
COMMENT     Sequence Label: Clover-Geminin(1-110)
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    71..104
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(143..472)
                     /codon_start=1
                     /product="degron consisting of residues 1-110 of human 
                     Geminin (Zielke and Edgar, 2015)"
                     /label=Gem1 (1-110)
                     /translation="MNPSMKQKQEEIKENIKNSSVPRRTLKMIQPSASGSLVGRENELS
                     AGLSKRKHRNDHLTSTTSSPGVIVPESSENKNLGGVTQESFDLMIKENPSSQYWKEVAE
                     KRRKAL"
     primer_bind     complement(545..566)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atccagaggt tgattatcga taagcttgat atcgaattgt acctagtgga accggaaccc
       61 ttaaacatgt ataacttcgt ataatgtatg ctatacgaag ttattaggtc cctcgacgaa
      121 ttccctgcag ggtttaaact tacagcgcct ttctccgttt ttctgccact tccttccaat
      181 actgagagga tggattttct ttaatcataa gatcaaatga ctcctgggtg actcctccaa
      241 gatttttatt ttcactagat tctgggacaa taaccccagg gctggaagtt gtagatgtta
      301 agtggtcatt ccgatgtttc cttttggaca agcctgcgga cagctcattt tctcttccaa
      361 caagagatcc agatgcagaa ggctgaatca tcttcagagt tcttcttggg acagaactat
      421 tctttatatt ctctttgatt tcttcttgtt tctgcttcat actgggattc atctcgagcg
      481 gccgccagtg tgcatggata tccttgtaca gctcgtccat gccatgtgta atcccggcgg
      541 cggtcacgaa ctccagcagg accatgtgat cgcgcttctc gttggggtct ttgctcaggg
      601 cggactgatg gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg
      661 tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc ctcaacgttg tggcggatct
      721 tgaagttagc cttgatgccg ttcttctgct tgtcggccgt gatatagacg ttgtggctgt
      781 tgaagttgta ctccagcttg tgcc
//
