LOCUS       sequence-164920-        4221 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing PPARG , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4221)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4221)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_PPARG_WT
FEATURES             Location/Qualifiers
     source          1..4221
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1621..1720)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1737..1756)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1738..1756)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1761..1777)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1761..1777)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2208..2999
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3092..3680
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3581..3600
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3834..3851
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4010..4037)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4129..4215)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcat gaccatggtt gacacagaga tgccattctg gcccaccaac tttgggatca
      241 gctccgtgga tctctccgta atggaagacc actcccactc ctttgatatc aagcccttca
      301 ctactgttga cttctccagc atttctactc cacattacga agacattcca ttcacaagaa
      361 cagatccagt ggttgcagat tacaagtatg acctgaaact tcaagagtac caaagtgcaa
      421 tcaaagtgga gcctgcatct ccaccttatt attctgagaa gactcagctc tacaataagc
      481 ctcatgaaga gccttccaac tccctcatgg caattgaatg tcgtgtctgt ggagataaag
      541 cttctggatt tcactatgga gttcatgctt gtgaaggatg caagggtttc ttccggagaa
      601 caatcagatt gaagcttatc tatgacagat gtgatcttaa ctgtcggatc cacaaaaaaa
      661 gtagaaataa atgtcagtac tgtcggtttc agaaatgcct tgcagtgggg atgtctcata
      721 atgccatcag gtttgggcgg atgccacagg ccgagaagga gaagctgttg gcggagatct
      781 ccagtgatat cgaccagctg aatccagagt ccgctgacct ccgggccctg gcaaaacatt
      841 tgtatgactc atacataaag tccttcccgc tgaccaaagc aaaggcgagg gcgatcttga
      901 caggaaagac aacagacaaa tcaccattcg ttatctatga catgaattcc ttaatgatgg
      961 gagaagataa aatcaagttc aaacacatca cccccctgca ggagcagagc aaagaggtgg
     1021 ccatccgcat ctttcagggc tgccagtttc gctccgtgga ggctgtgcag gagatcacag
     1081 agtatgccaa aagcattcct ggttttgtaa atcttgactt gaacgaccaa gtaactctcc
     1141 tcaaatatgg agtccacgag atcatttaca caatgctggc ctccttgatg aataaagatg
     1201 gggttctcat atccgagggc caaggcttca tgacaaggga gtttctaaag agcctgcgaa
     1261 agccttttgg tgactttatg gagcccaagt ttgagtttgc tgtgaagttc aatgcactgg
     1321 aattagatga cagcgacttg gcaatattta ttgctgtcat tattctcagt ggagaccgcc
     1381 caggtttgct gaatgtgaag cccattgaag acattcaaga caacctgcta caagccctgg
     1441 agctccagct gaagctgaac caccctgagt cctcacagct gtttgccaag ctgctccaga
     1501 aaatgacaga cctcagacag attgtcacgg aacacgtgca gctactgcag gtgatcaaga
     1561 agacggagac agacatgagt cttcacccgc tcctgcagga gatctacaag gacttgtact
     1621 acccaacttt cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca
     1681 acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct gatatcccct
     1741 atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa
     1801 atctctgatg ttacattgca caagataaaa atatatcatc atgcctcctc tagaccagcc
     1861 aggacagaaa tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc acaccgtgga
     1921 aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca
     1981 agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg
     2041 gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc
     2101 gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca
     2161 acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacattatg agggaagcgg
     2221 tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg
     2281 aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac
     2341 acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag
     2401 ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg
     2461 ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc
     2521 gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag
     2581 ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct
     2641 tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg
     2701 cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa
     2761 atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga
     2821 aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac
     2881 ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc
     2941 agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataac
     3001 cctcgagcca cccatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt
     3061 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     3121 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     3181 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     3241 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     3301 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     3361 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     3421 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     3481 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     3541 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     3601 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     3661 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     3721 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     3781 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     3841 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     3901 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     3961 acgcaattaa tacgcgtacc gctagccagg aagagtttgt agaaacgcaa aaaggccatc
     4021 cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc gtcctgcccg
     4081 ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat ttgtcctact
     4141 caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt ccgactgagc
     4201 ctttcgtttt atttgatgcc t
//
