LOCUS       sequence-164922-        5851 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing PRKCA , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5851)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 5851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_PRKCA_WT
FEATURES             Location/Qualifiers
     source          1..5851
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(156..183)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(275..361)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     425..441
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    544..568
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2588..2687)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2704..2723)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2705..2723)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2728..2744)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2728..2744)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3175..3966
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4059..4647
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4548..4567
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4801..4818
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4977..5004)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5096..5182)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     5246..5262
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5278..5377
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
       61 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatacg
      121 cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct
      181 tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc
      241 gttgcttcac aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca
      301 ccgacaaaca acagataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattt
      361 gatgcctggc agttccctac tctcgcgtta acgctagcat ggatgttttc ccagtcacga
      421 cgttgtaaaa cgacggccag tcttaagctc gggccccaaa taatgatttt attttgactg
      481 atagtgacct gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg
      541 gggacaactt tgtacaaaaa agttggcacc atggctgacg ttttcccggg caacgactcc
      601 acggcgtctc aggacgtggc caaccgcttc gcccgcaaag gggcgctgag gcagaagaac
      661 gtgcacgagg tgaaggacca caaattcatc gcgcgcttct tcaagcagcc caccttctgc
      721 agccactgca ccgacttcat ctgggggttt gggaaacaag gcttccagtg ccaagtttgc
      781 tgttttgtgg tccacaagag gtgccatgaa tttgttactt tttcttgtcc gggtgcggat
      841 aagggacccg acactgatga ccccaggagc aagcacaagt tcaaaatcca cacttacgga
      901 agccccacct tctgcgatca ctgtgggtca ctgctctatg gacttatcca tcaagggatg
      961 aaatgtgaca cctgcgatat gaacgttcac aagcaatgcg tcatcaatgt ccccagcctc
     1021 tgcggaatgg atcacactga gaagaggggg cggatttacc taaaggctga ggttgctgat
     1081 gaaaagctcc atgtcacagt acgagatgca aaaaatctaa tccctatgga tccaaacggg
     1141 ctttcagatc cttatgtgaa gctgaaactt attcctgatc ccaagaatga aagcaagcaa
     1201 aaaaccaaaa ccatccgctc cacactaaat ccgcagtgga atgagtcctt tacattcaaa
     1261 ttgaaacctt cagacaaaga ccgacgactg tctgtagaaa tctgggactg ggatcgaaca
     1321 acaaggaatg acttcatggg atccctttcc tttggagttt cggagctgat gaagatgccg
     1381 gccagtggat ggtacaagtt gcttaaccaa gaagaaggtg agtactacaa cgtacccatt
     1441 ccggaagggg acgaggaagg aaacatggaa ctcaggcaga aattcgagaa agccaaactt
     1501 ggccctgctg gcaacaaagt catcagtccc tctgaagaca ggaaacaacc ttccaacaac
     1561 cttgaccgag tgaaactcac ggacttcaat ttcctcatgg tgttgggaaa ggggagtttt
     1621 ggaaaggtga tgcttgccga caggaagggc acagaagaac tgtatgcaat caaaatcctg
     1681 aagaaggatg tggtgattca ggatgatgac gtggagtgca ccatggtaga aaagcgagtc
     1741 ttggccctgc ttgacaaacc cccgttcttg acgcagctgc actcctgctt ccagacagtg
     1801 gatcggctgt acttcgtcat ggaatatgtc aacggtgggg acctcatgta ccacattcag
     1861 caagtaggaa aatttaagga accacaagca gtattctatg cggcagagat ttccatcgga
     1921 ttgttctttc ttcataaaag aggaatcatt tatagggatc tgaagttaga taacgtcatg
     1981 ttggattcag aaggacatat caaaattgct gactttggga tgtgcaagga acacatgatg
     2041 gatggagtca cgaccaggac cttctgtggg actccagatt atatcgcccc agagataatc
     2101 gcttatcagc cgtatggaaa atctgtggac tggtgggcct atggcgtcct gttgtatgaa
     2161 atgcttgccg ggcagcctcc atttgatggt gaagatgaag acgagctatt tcagtctatc
     2221 atggagcaca acgtttccta tccaaaatcc ttgtccaagg aggctgtttc tatctgcaaa
     2281 ggactgatga ccaaacaccc agccaagcgg ctgggctgtg ggcctgaggg ggagagggac
     2341 gtgagagagc atgccttctt ccggaggatc gactgggaaa aactggagaa cagggagatc
     2401 cagccaccat tcaagcccaa agtgtgtggc aaaggagcag agaactttga caagttcttc
     2461 acacgaggac agcccgtctt aacaccacct gatcagctgg ttattgctaa catagaccag
     2521 tctgattttg aagggttctc gtatgtcaac ccccagtttg tgcaccccat cttacagagt
     2581 gcagtattgc caactttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt
     2641 tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat
     2701 atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg
     2761 tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg cctcctctag
     2821 accagccagg acagaaatgc ctcgacttcg ctgctaccca aggttgccgg gtgacgcaca
     2881 ccgtggaaac ggatgaaggc acgaacccag tggacataag cctgttcggt tcgtaagctg
     2941 taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt
     3001 ggtaacggcg cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct
     3061 atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg
     3121 agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa cattatgagg
     3181 gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc
     3241 catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg
     3301 aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg
     3361 cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt
     3421 ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca
     3481 gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc
     3541 gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc
     3601 gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta
     3661 tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat
     3721 gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc
     3781 gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc
     3841 gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc
     3901 gcagatcagt tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc
     3961 aaataaccct cgagccaccc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac
     4021 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     4081 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     4141 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     4201 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     4261 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     4321 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     4381 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     4441 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     4501 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     4561 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     4621 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     4681 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     4741 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     4801 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     4861 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     4921 gagcgcaacg caattaatac gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa
     4981 ggccatccgt caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc
     5041 ctgcccgcca ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg
     5101 tcctactcag gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg
     5161 actgagcctt tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt aacgctagca
     5221 tggatgtttt cccagtcacg acgttgtaaa acgacggcca gtcttaagct cgggccccaa
     5281 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga tgagcaatgc
     5341 ttttttataa tgccaacttt gtacaaaaaa gttggcacca tggctgacgt tttcccgggc
     5401 aacgactcca cggcgtctca ggacgtggcc aaccgcttcg cccgcaaagg ggcgctgagg
     5461 cagaagaacg tgcacgaggt gaaggaccac aaattcatcg cgcgcttctt caagcagccc
     5521 accttctgca gccactgcac cgacttcatc tgggggtttg ggaaacaagg cttccagtgc
     5581 caagtttgct gttttgtggt ccacaagagg tgccatgaat ttgttacttt ttcttgtccg
     5641 ggtgcggata agggacccga cactgatgac cccaggagca agcacaagtt caaaatccac
     5701 acttacggaa gccccacctt ctgcgatcac tgtgggtcac tgctctatgg acttatccat
     5761 caagggatga aatgtgacac ctgcgatatg aacgttcaca agcaatgcgt catcaatgtc
     5821 cccagcctct gcggaatgga tcacactgag a
//
