LOCUS       sequence-164923-        4809 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing PRKCB , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_PRKCB_WT
FEATURES             Location/Qualifiers
     source          1..4809
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2209..2308)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2325..2344)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2326..2344)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2349..2365)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2349..2365)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2796..3587
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3680..4268
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4169..4188
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4422..4439
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4598..4625)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4717..4803)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaat
      181 aagttggcat ggctgacccg gctgcggggc cgccgccgag cgagggcgag gagagcaccg
      241 tgcgcttcgc ccgcaaaggc gccctccggc agaagaacgt gcatgaggtc aagaaccaca
      301 aattcaccgc ccgcttcttc aagcagccca ccttctgcag ccactgcacc gacttcatct
      361 ggggcttcgg gaagcaggga ttccagtgcc aagtttgctg ctttgtggtg cacaagcggt
      421 gccatgaatt tgtcacattc tcctgccctg gcgctgacaa gggtccagcc tccgatgacc
      481 cccgcagcaa acacaagttt aagatccaca cgtactccag ccccacgttt tgtgaccact
      541 gtgggtcact gctgtatgga ctcatccacc aggggatgaa atgtgacacc tgcatgatga
      601 atgtgcacaa gcgctgcgtg atgaatgttc ccagcctgtg tggcacggac cacacggagc
      661 gccgcggccg catctacatc caggcccaca tcgacaggga cgtcctcatt gtcctcgtaa
      721 gagatgctaa aaaccttgta cctatggacc ccaatggcct gtcagatccc tacgtaaaac
      781 tgaaactgat tcccgatccc aaaagtgaga gcaaacagaa gaccaaaacc atcaaatgct
      841 ccctcaaccc tgagtggaat gagacattta gatttcagct gaaagaatcg gacaaagaca
      901 gaagactgtc agtagagatt tgggattggg atttgaccag caggaatgac ttcatgggat
      961 ctttgtcctt tgggatttct gaacttcaga aagccagtgt tgatggctgg tttaagttac
     1021 tgagccagga ggaaggcgag tacttcaatg tgcctgtgcc accagaagga agtgaggcca
     1081 atgaagaact gcggcagaaa tttgagaggg ccaagatcag tcagggaacc aaggtcccgg
     1141 aagaaaagac gaccaacact gtctccaaat ttgacaacaa tggcaacaga gaccggatga
     1201 aactgaccga ttttaacttc ctaatggtgc tggggaaagg cagctttggc aaggtcatgc
     1261 tttcagaacg aaaaggcaca gatgagctct atgctgtgaa gatcctgaag aaggacgttg
     1321 tgatccaaga tgatgacgtg gagtgcacta tggtggagaa gcgggtgttg gccctgcctg
     1381 ggaagccgcc cttcctgacc cagctccact cctgcttcca gaccatggac cgcctgtact
     1441 ttgtgatgga gtacgtgaat gggggcgacc tcatgtatca catccagcaa gtcggccggt
     1501 tcaaggagcc ccatgctgta ttttacgctg cagaaattgc catcggtctg ttcttcttac
     1561 agagtaaggg catcatttac cgtgacctaa aacttgacaa cgtgatgctc gattctgagg
     1621 gacacatcaa gattgccgat tttggcatgt gtaaggaaaa catctgggat ggggtgacaa
     1681 ccaagacatt ctgtggcact ccagactaca tcgcccccga gataattgct tatcagccct
     1741 atgggaagtc cgtggattgg tgggcatttg gagtcctgct gtatgaaatg ttggctgggc
     1801 aggcaccctt tgaaggggag gatgaagatg aactcttcca atccatcatg gaacacaacg
     1861 tagcctatcc caagtctatg tccaaggaag ctgtggccat ctgcaaaggg ctgatgacca
     1921 aacacccagg caaacgtctg ggttgtggac ctgaaggcga acgtgatatc aaagagcatg
     1981 catttttccg gtatattgat tgggagaaac ttgaacgcaa agagatccag cccccttata
     2041 agccaaaagc ttgtgggcga aatgctgaaa acttcgaccg atttttcacc cgccatccac
     2101 cagtcctaac acctcccgac caggaagtca tcaggaatat tgaccaatca gaattcgaag
     2161 gattttcctt tgttaactct gaatttttaa aacccgaagt caagagctac ccaactttct
     2221 tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca
     2281 ctatcagtca aaataaaatc attatttgcc atccagctga tatcccctat agtgagtcgt
     2341 attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt
     2401 acattgcaca agataaaaat atatcatcat gcctcctcta gaccagccag gacagaaatg
     2461 cctcgacttc gctgctaccc aaggttgccg ggtgacgcac accgtggaaa cggatgaagg
     2521 cacgaaccca gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc
     2581 gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg
     2641 ttttcatggc ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc
     2701 agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc
     2761 agcagggcag tcgccctaaa acaaagttaa acattatgag ggaagcggtg atcgccgaag
     2821 tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc
     2881 tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg
     2941 atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg
     3001 accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca
     3061 ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat
     3121 ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca
     3181 ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag
     3241 cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa
     3301 ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta
     3361 cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg
     3421 ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac
     3481 aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat
     3541 ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg caaataaccc tcgagccacc
     3601 catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag
     3661 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     3721 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     3781 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     3841 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     3901 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     3961 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     4021 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     4081 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     4141 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     4201 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     4261 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     4321 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     4381 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     4441 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     4501 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata
     4561 cgcgtaccgc tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc
     4621 cttctgctta gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg
     4681 ccgttgcttc acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt
     4741 caccgacaaa caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat
     4801 ttgatgcct
//
