LOCUS       sequence-164924-        1022 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid containing a P2A peptide (porcine teschovirus-1 derived) 
            sequence that works efficiently in Saccharomyces cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   DiSanto R, Aboulhouda S, Weinberg DE
  TITLE     The fail-safe mechanism of post-transcriptional silencing of 
            unspliced HAC1 mRNA.
  JOURNAL   Elife. 2016 Oct 1;5. pii: e20069. doi: 10.7554/eLife.20069.
  PUBMED    27692069
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-10-1"; volume: "5. pii: e20069. doi"; pages: " 
            10.7554/eLife.20069"
COMMENT     Sequence Label: pDEW8
FEATURES             Location/Qualifiers
     source          1..1022
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             92..118
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             128..835
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="VSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTMR
                     IKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGGV
                     VTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTHM
                     ALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAVA
                     KFAGLGGGMDELYK"
     CDS             878..934
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 tttcttaaac ttcttaaatt ctacttttat agttagtctt ttttttagtt ttaaaacacc
       61 agaacttagt ttcgacggat ctagaactat gtacccatac gatgttcctg actatgcggg
      121 ttctggtgtg tccaaaggag aggagttaat caaggaaaac atgagaatga aagttgtcat
      181 ggagggctcc gttaatggtc accaattcaa gtgtacaggg gaaggtgaag gtaatcctta
      241 catgggtaca caaactatga gaattaaagt aattgaaggc ggaccactac catttgcatt
      301 tgacattctg gcaacgtcat tcatgtacgg atcacgaact ttcatcaagt accctaaagg
      361 tataccagac tttttcaagc aatcttttcc agagggtttt acatgggaaa gggttacaag
      421 atacgaagat gggggtgtcg tcacagttat gcaagatact tcattagaag atggctgcct
      481 tgtctatcat gtgcaagtaa gaggggtgaa ttttccttct aacggacctg tgatgcagaa
      541 aaagaccaaa ggttgggaac caaatactga aatgatgtac ccagctgatg gaggtttgag
      601 aggctacaca cacatggcgc ttaaagttga tggtggaggt catttgtctt gtagttttgt
      661 taccacttat cgttctaaaa agactgttgg caatatcaaa atgccaggaa tacatgctgt
      721 agaccacaga ctagaaagac tcgaagagag cgataacgaa atgttcgttg tacagagaga
      781 gcatgccgta gccaaatttg ctggcttagg cggtggtatg gatgaattgt ataagggttc
      841 aggagctatg actgtgatga cattccaggg accaggtgca acaaacttct ccctcttgaa
      901 acaagcagga gatgttgagg aaaatcccgg gcctatgtca aaaggcgagg aactattcac
      961 aggtgtcgta ccaatactgg tagaattgga tggcgacgta aacgggcata agttttctgt
     1021 ga
//
