LOCUS       sequence-164927-       11391 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral expression vector for MS2-AID fusion protein with 
            hygromycin B selectable marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11391)
  AUTHORS   Hess GT, Fresard L, Han K, Lee CH, Li A, Cimprich KA, Montgomery SB,
            Bassik MC
  TITLE     Directed evolution using dCas9-targeted somatic hypermutation in 
            mammalian cells.
  JOURNAL   Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038.
  PUBMED    27798611
REFERENCE   2  (bases 1 to 11391)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038."
COMMENT     Sequence Label: pGH156_MS2-AID-Hygro
FEATURES             Location/Qualifiers
     source          1..11391
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2687..3865
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2918..3856
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3813..3833
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3872..3881
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3881..4267
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             4322..4342
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4358..4951
                     /codon_start=1
                     /gene="AICDA"
                     /product="human activation-induced cytidine deaminase 
                     (Dickerson et al., 2003)"
                     /label=AID
                     /translation="MDSLLMNRRKFLYQFKNVRWAKGRRETYLCYVVKRRDSATSFSLD
                     FGYLRNKNGCHVELLFLRYISDWDLDPGRCYRVTWFTSWSPCYDCARHVADFLRGNPNL
                     SLRIFTARLYFCEDRKAEPEGLRRLHRAGVQIAIMTFKDYFYCWNTFVENHERTFKAWE
                     GLHENSVRLSRQLRRILLPLYEVDDLRDAFRTLGL"
     CDS             4967..5020
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5024..6043
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     "
     misc_feature    6083..6671
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6136..6156)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6554..6565)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6674..6690)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6675..6691)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7196..7376
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7402..7419)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7408..7632
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7678..8106
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7765..7784)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7975..7996
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8115..8135)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8120..8449
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8300..8435
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8362..8381
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8497..8544
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8563..8937
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9067..9188
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9104..9123)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9158..9177
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9237..9253)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9237..9253)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9250..9272)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9261..9277
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9285..9291,9292..9309,9310..9315))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9330..9351
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9468..9485)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9639..10227)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9719..9738)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10398..11189,11190..11258))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11021..11040
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11259..11363)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta gctagctgca aagatggata aagttttaaa cagagaggaa
     2641 tctttgcagc taatggacct tctaggtctt gaaaggagtg ggaattggct ccggtgcccg
     2701 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa
     2761 ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg
     2821 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa
     2881 cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg
     2941 cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct
     3001 gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc
     3061 ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg
     3121 ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct
     3181 agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt
     3241 aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg
     3301 ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag
     3361 aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc
     3421 gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga
     3481 aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg
     3541 agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc
     3601 ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt
     3661 ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac
     3721 tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt
     3781 tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt
     3841 ttttcttcca tttcaggtgt cgtgacgtac ggccaccatg gcttcaaact ttactcagtt
     3901 cgtgctcgtg gacaatggtg ggacagggga tgtgacagtg gctccttcta atttcgctaa
     3961 tggggtggca gagtggatca gctccaactc acggagccag gcctacaagg tgacatgcag
     4021 cgtcaggcag tctagtgccc agaagagaaa gtataccatc aaggtggagg tccccaaagt
     4081 ggctacccag acagtgggcg gagtcgaact gcctgtcgcc gcttggaggt cctacctgaa
     4141 catggagctc actatcccaa ttttcgctac caattctgac tgtgaactca tcgtgaaggc
     4201 aatgcagggg ctcctcaaag acggtaatcc tatcccttcc gccatcgccg ctaactcagg
     4261 tatctacagc gctggaggag gtggaagcgg aggaggagga agcggaggag gaggtagcgg
     4321 acctaagaaa aagaggaagg tggcggccgc tggatccatg gacagcctct tgatgaaccg
     4381 gaggaagttt ctttaccaat tcaaaaatgt ccgctgggct aagggtcggc gtgagaccta
     4441 cctgtgctac gtagtgaaga ggcgtgacag tgctacatcc ttttcactgg actttggtta
     4501 tcttcgcaat aagaacggct gccacgtgga attgctcttc ctccgctaca tctcggactg
     4561 ggacctagac cctggccgct gctaccgcgt cacctggttc acctcctgga gcccctgcta
     4621 cgactgtgcc cgacatgtgg ccgactttct gcgagggaac cccaacctca gtctgaggat
     4681 cttcaccgcg cgcctctact tctgtgagga ccgcaaggct gagcccgagg ggctgcggcg
     4741 gctgcaccgc gccggggtgc aaatagccat catgaccttc aaagattatt tttactgctg
     4801 gaatactttt gtagaaaacc acgaaagaac tttcaaagcc tgggaagggc tgcatgaaaa
     4861 ttcagttcgt ctctccagac agcttcggcg catccttttg cccctgtatg aggttgatga
     4921 cttacgagac gcatttcgta ctttgggact ttgtacaggc agtggagagg gcagaggaag
     4981 tctgctaaca tgcggtgacg tcgaggagaa tcctggccca accatgaaaa agcctgaact
     5041 caccgctacc tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat
     5101 gcagctctcc gagggcgaag aatctcgggc tttcagcttc gatgtgggag ggcgtggata
     5161 tgtcctgcgg gtgaatagct gcgccgatgg tttctacaaa gatcgctatg tttatcggca
     5221 ctttgcatcc gccgctctcc ctattcccga agtgcttgac attggggagt tcagcgagag
     5281 cctgacctat tgcatctccc gccgtgcaca gggtgtcacc ttgcaagacc tgcctgaaac
     5341 cgaactgccc gctgttctcc agcccgtcgc cgaggccatg gatgccatcg ctgccgccga
     5401 tcttagccag accagcgggt tcggcccatt cggacctcaa ggaatcggtc aatacactac
     5461 atggcgcgat ttcatctgcg ctattgctga tccccatgtg tatcactggc aaactgtgat
     5521 ggacgacacc gtcagtgcct ccgtcgccca ggctctcgat gagctgatgc tttgggccga
     5581 ggactgcccc gaagtccggc acctcgtgca cgccgatttc ggctccaaca atgtcctgac
     5641 cgacaatggc cgcataacag ccgtcattga ctggagcgag gccatgttcg gggattccca
     5701 atacgaggtc gccaacatct tcttctggag gccctggttg gcttgtatgg agcagcagac
     5761 ccgctacttc gagcggaggc atcccgagct tgcaggatct cctcggctcc gggcttatat
     5821 gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc
     5881 agcttgggct cagggtcgct gcgacgcaat cgtccggtcc ggagccggga ctgtcgggcg
     5941 tacacaaatc gcccgcagaa gcgctgccgt ctggaccgat ggctgtgtgg aagtgctcgc
     6001 cgatagtgga aacagacgcc ccagcactcg tcctagggca aaggatctgc agtaatgaga
     6061 attcgatatc aagcttatcg gtaatcaacc tctggattac aaaatttgtg aaagattgac
     6121 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     6181 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     6241 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     6301 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     6361 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     6421 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     6481 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     6541 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     6601 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     6661 cgcctccccg catcgatacc gtcgacctcg agacctagaa aaacatggag caatcacaag
     6721 tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga
     6781 ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt
     6841 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     6901 aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca
     6961 gaactacaca ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct
     7021 agtaccagtt gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt
     7081 acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt
     7141 tgacagccgc ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc
     7201 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     7261 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     7321 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc
     7381 ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     7441 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     7501 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     7561 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     7621 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
     7681 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     7741 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     7801 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     7861 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     7921 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     7981 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     8041 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     8101 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     8161 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     8221 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     8281 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     8341 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
     8401 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
     8461 agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag
     8521 tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt
     8581 gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg
     8641 ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg
     8701 accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg
     8761 tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc
     8821 cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc
     8881 gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac
     8941 gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     9001 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     9061 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     9121 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     9181 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     9241 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     9301 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     9361 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     9421 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     9481 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     9541 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     9601 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     9661 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     9721 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     9781 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     9841 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     9901 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     9961 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
    10021 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
    10081 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
    10141 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
    10201 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
    10261 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
    10321 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
    10381 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
    10441 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
    10501 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
    10561 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
    10621 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
    10681 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
    10741 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
    10801 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
    10861 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
    10921 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
    10981 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
    11041 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
    11101 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
    11161 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
    11221 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
    11281 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
    11341 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga c
//
