LOCUS       sequence-164938-        5004 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing PRKCE , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5004)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 5004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_PRKCE_WT
FEATURES             Location/Qualifiers
     source          1..5004
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             839..850
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="NHKV"
     protein_bind    complement(2404..2503)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2520..2539)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2521..2539)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2544..2560)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2544..2560)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2991..3782
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3875..4463
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4364..4383
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4617..4634
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4793..4820)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4912..4998)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catggtagtg ttcaatggcc ttcttaagat caaaatctgc gaggccgtga
      241 gcttgaagcc cacagcctgg tcgctgcgcc atgcggtggg accccggccg cagactttcc
      301 ttctcgaccc ctacattgcc ctcaatgtgg acgactcgcg catcggccaa acggccacca
      361 agcagaagac caacagcccg gcctggcacg acgagttcgt caccgatgtg tgcaacggac
      421 gcaagatcga gctggctgtc tttcacgatg cccccatagg ctacgacgac ttcgtggcca
      481 actgcaccat ccagtttgag gagctgctgc agaacgggag ccgccacttc gaggactgga
      541 ttgatctgga gccagaagga agagtgtatg tgatcatcga tctctcaggg tcgtcgggtg
      601 aagcccctaa agacaatgaa gagcgtgtgt tcagggaacg catgcggccg aggaagcggc
      661 agggggccgt caggcgcagg gtccatcagg tcaacggcca caagttcatg gccacctatc
      721 ttcggcagcc cacctactgc tcccattgca gagacttcat ctggggtgtc ataggaaagc
      781 agggatacca gtgtcaagtc tgcacctgcg tggtccacaa gcggtgccac gagctcataa
      841 tcacaaagtg tgctgggtta aagaagcagg agacccccga ccaggtgggc tcccagcggt
      901 tcagcgtcaa catgccccac aagttcggta tccacaacta caaggtccct accttctgcg
      961 atcactgtgg gtccctgctc tggggactct tgcggcaggg tttgcagtgt aaagtctgca
     1021 aaatgaatgt tcaccgtcga tgtgagacca acgtggctcc caactgtgga gtggatgcca
     1081 gaggaatcgc caaagtactg gccgacctgg gcgttacccc agacaaaatc accaacagcg
     1141 gccagagaag gaaaaagctc attgctggtg ccgagtcccc gcagcctgct tctggaagct
     1201 caccatctga ggaagatcga tccaagtcag cacccacctc cccttgtgac caggaaataa
     1261 aagaacttga gaacaacatt cggaaagcct tgtcatttga caaccgagga gaggagcacc
     1321 gggcagcatc gtctcctgat ggccagctga tgagccccgg tgagaatggc gaagtccggc
     1381 aaggccaggc caagcgcctg ggcctggatg agttcaactt catcaaggtg ttgggcaaag
     1441 gcagctttgg caaggtcatg ttggcagaac tcaagggcaa agatgaagta tatgctgtga
     1501 aggtcttaaa gaaggacgtc atccttcagg atgatgacgt ggactgcaca atgacagaga
     1561 agaggatttt ggctctggca cggaaacacc cgtaccttac ccaactctac tgctgcttcc
     1621 agaccaagga ccgcctcttt ttcgtcatgg aatatgtaaa tggtggagac ctcatgtttc
     1681 agattcagcg ctcccgaaaa ttcgacgagc ctcgttcacg gttctatgct gcagaggtca
     1741 catcggccct catgttcctc caccagcatg gagtcatcta cagggatttg aaactggaca
     1801 acatccttct ggatgcagaa ggtcactgca agctggctga cttcgggatg tgcaaggaag
     1861 ggattctgaa tggtgtgacg accaccacgt tctgtgggac tcctgactac atagctcctg
     1921 agatcctgca ggagttggag tatggcccct ccgtggactg gtgggccctg ggggtgctga
     1981 tgtacgagat gatggctgga cagcctccct ttgaggccga caatgaggac gacctatttg
     2041 agtccatcct ccatgacgac gtgctgtacc cagtctggct cagcaaggag gctgtcagca
     2101 tcttgaaagc tttcatgacg aagaatcccc acaagcgcct gggctgtgtg gcatcgcaga
     2161 atggcgagga cgccatcaag cagcacccat tcttcaaaga gattgactgg gtgctcctgg
     2221 agcagaagaa gatcaagcca cccttcaaac cacgcattaa aaccaaaaga gacgtcaata
     2281 attttgacca agactttacc cgggaagagc cggtactcac ccttgtggac gaagcaattg
     2341 taaagcagat caaccaggag gaattcaaag gtttctccta ctttggtgaa gacctgatgc
     2401 ccttgccaac tttcttgtac aaagttggca ttataagaaa gcattgctta tcaatttgtt
     2461 gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgccatcca gctgatatcc
     2521 cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc
     2581 aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgcctc ctctagacca
     2641 gccaggacag aaatgcctcg acttcgctgc tacccaaggt tgccgggtga cgcacaccgt
     2701 ggaaacggat gaaggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat
     2761 gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta
     2821 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc
     2881 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
     2941 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatt atgagggaag
     3001 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc
     3061 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc
     3121 cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc
     3181 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc
     3241 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta
     3301 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc
     3361 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg
     3421 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg
     3481 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc
     3541 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc
     3601 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca
     3661 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag
     3721 atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat
     3781 aaccctcgag ccacccatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag
     3841 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     3901 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     3961 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     4021 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     4081 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     4141 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     4201 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     4261 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     4321 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     4381 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     4441 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     4501 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     4561 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     4621 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     4681 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     4741 gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg caaaaaggcc
     4801 atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg ggcgtcctgc
     4861 ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg gatttgtcct
     4921 actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt cttccgactg
     4981 agcctttcgt tttatttgat gcct
//
