LOCUS       sequence-16495-e         957 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Glover JR, Kowal AS, Schirmer EC, Patino MM, Liu JJ, Lindquist S
  TITLE     Self-seeded fibers formed by Sup35, the protein determinant of 
            [PSI+], a heritable prion-like factor of S. cerevisiae.
  JOURNAL   Cell 1997 May 30;89(5):811-9.
  PUBMED    9182769
REFERENCE   2  (bases 1 to 957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell"; 
            date: "1997-05-30"; volume: "89(5)"; pages: "811-9"
COMMENT     Sequence Label: pRSCupGFP
FEATURES             Location/Qualifiers
     source          1..957
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     29..45
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     30..46
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        467..701
                     /gene="S. cerevisiae CUP1"
                     /label=CUP1 promoter
                     /note="inducible metallothionein promoter, regulated by 
                     Cu2+"
     primer_bind     complement(792..820)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ggaacaaaag ctggtaccgg gccccccctc gaggtcgacg gtatcgataa gcttgatatc
       61 gaatttccca ttaccgacat ttgggcggcg gccaaagcgg tcggacagtg ctccgagaac
      121 gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc
      181 gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc
      241 tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt
      301 catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagcta
      361 aaacactttt gtattatttt tcctcatata tgtgtatagg tttatacgga tgatttaatt
      421 attacttcac caccctttat ttcaggctga tatcttagcc ttgttactag ttagaaaaag
      481 acatttttgc tgtcagtcac tgtcaagaga ttcttttgct ggcatttctt ctagaagcaa
      541 aaagagcgat gcgtcttttc cgctgaaccg ttccagcaaa aaagactacc aacgcaatat
      601 ggattgtcag aatcatataa aagagaagca aataactcct tgtcttgtat caattgcatt
      661 ataatatctt cttgttagtg caatatcata tagaagtcat cgaaatagat attaagaaaa
      721 acaaactgta caatcaatca atcaatcagg atccccatat ggctagcaaa ggagaagaac
      781 tcttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat
      841 tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaatttat
      901 ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactt tcactta
//
