LOCUS       sequence-164970-        1014 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid containing a P2A peptide (porcine teschovirus-1 derived) 
            sequence that works efficiently in Saccharomyces cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   DiSanto R, Aboulhouda S, Weinberg DE
  TITLE     The fail-safe mechanism of post-transcriptional silencing of 
            unspliced HAC1 mRNA.
  JOURNAL   Elife. 2016 Oct 1;5. pii: e20069. doi: 10.7554/eLife.20069.
  PUBMED    27692069
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-10-1"; volume: "5. pii: e20069. doi"; pages: " 
            10.7554/eLife.20069"
COMMENT     Sequence Label: pDEW8
FEATURES             Location/Qualifiers
     source          1..1014
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..26
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7..25
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(44..291)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     257..275
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     293..309
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     294..310
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgcgcgtaat acgactcact atagggcgaa ttgggtaccg gccgcaaatt aaagccttcg
       61 agcgtcccaa aaccttctca agcaaggttt tcagtataat gttacatgcg tacacgcgtc
      121 tgtacagaaa aaaaagaaaa atttgaaata taaataacgt tcttaatact aacataacta
      181 taaaaaaata aatagggacc tagacttcag gttgtctaac tccttccttt tcggttagag
      241 cggatgtggg gggagggcgt gaatgtaagc gtgacataac taattacatg actcgaggtc
      301 gacggtatcg attcacttgt ataattcatc catgcccaac gttatacccg cagcggtgac
      361 aaattctaac aaaaccatgt gatctctctt ttcattagga tcttttgaca aggcgctctg
      421 tgtagataag taatggttgt ctggcaatag aacaggtcca tctccgattg gtgtattttg
      481 ttgataatga tcagcgagtt gaacggaacc atcttcgata ttatgtctaa ttttgaaatt
      541 aacttttatg ccgtttttct gtttatctgc catgatgtaa acgttatgtg aattgtagtt
      601 gtattccaat ttgtgaccta agatattacc atcttcctta aagtcaatac cctttaactc
      661 aattctatta acaagtgtat ctccttcaaa cttcacttca gcacgagttt tgtaattacc
      721 atcatcttta aagaaaatag ttctctcttg cacatatccc tcaggcatgg cagatttgaa
      781 aaagtcatgt tgtttcatgt ggtctgggta tcgagaaaaa cattgtactc cgtaagtgag
      841 agttgtaact agagttggcc atgggacagg cagcttccct gtggtgcaga tgaacttaag
      901 tgtaagtttg ccataagttg cgtccccttc accctcacca ctcacagaaa acttatgccc
      961 gtttacgtcg ccatccaatt ctaccagtat tggtacgaca cctgtgaata gttc
//
