LOCUS       sequence-164971-        1015 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid containing a P2A peptide (porcine teschovirus-1 derived) 
            sequence that works efficiently in Saccharomyces cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   DiSanto R, Aboulhouda S, Weinberg DE
  TITLE     The fail-safe mechanism of post-transcriptional silencing of 
            unspliced HAC1 mRNA.
  JOURNAL   Elife. 2016 Oct 1;5. pii: e20069. doi: 10.7554/eLife.20069.
  PUBMED    27692069
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-10-1"; volume: "5. pii: e20069. doi"; pages: " 
            10.7554/eLife.20069"
COMMENT     Sequence Label: pDEW8
FEATURES             Location/Qualifiers
     source          1..1015
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(208..395)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     primer_bind     complement(542..561)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     661..683
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(721..739)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cagagcatgt atcatatggt ccagaaaccc tatacctgtg tggacgttaa tcacttgcga
       61 ttgtgtggcc tgttctgcta ctgcttctgc ctctttttct gggaagatcg agtgctctat
      121 cgctagggga ccacccttta aagagatcgc aatctgaatc ttggtttcat ttgtaatacg
      181 ctttactagg gctttctgct ctgtcatctt tgccttcgtt tatcttgcct gctcattttt
      241 tagtatattc ttcgaagaaa tcacattact ttatataatg tataattcat tatgtgataa
      301 tgccaatcgc taagaaaaaa aaagagtcat ccgctaggtg gaaaaaaaaa aatgaaaatc
      361 attaccgagg cataaaaaaa tatagagtgt actagaggag gccaagagta atagaaaaag
      421 aaaattgcgg gaaaggactg tgttatgact tccctgacta atgccgtgtt caaacgatac
      481 ctggcagtga ctcctagcgc tcaccaagct cttaaaacgg gaatttatgg tgcactctca
      541 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
      601 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
      661 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
      721 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagggtc
      781 cttttcatca cgtgctataa aaataattat aatttaaatt ttttaatata aatatataaa
      841 ttaaaaatag aaagtaaaaa aagaaattaa agaaaaaata gtttttgttt tccgaagatg
      901 taaaagactc tagggggatc gccaacaaat actacctttt atcttgctct tcctgctctc
      961 aggtattaat gccgaattgt ttcatcttgt ctgtgtagaa gaccacacac gaaaa
//
