LOCUS       sequence-16503-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Schirmer EC, Ware DM, Queitsch C, Kowal AS, Lindquist SL
  TITLE     Subunit interactions influence the biochemical and biological 
            properties of Hsp104.
  JOURNAL   Proc Natl Acad Sci U S A 2001 Jan 30;98(3):914-9. Epub 2001 Jan 23.
  PUBMED    11158570
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A"; date: "2001-01-30"; volume: "98(3)"; pages: "914-9.
            Epub 2001 Jan 23"
COMMENT     Sequence Label: K218Tc-3
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(61..77)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(61..77)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(704..720)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(704..720)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(717..739)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    728..744
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(752..758,759..776,777..781))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 gcgggccccc ngaggtcgac ggtatcgata agcttgatat cgaattcctg cagcccgggg
       61 gatccactag ttctagagcg gccgtgaaga agaaataaga agcactatta gagttttagc
      121 aagaagaatt aagtccaacc catgtttaat tggtgagcca ggtatcggta ccaccgctat
      181 tattgaaggt gttgctcaaa gaatcattga cgatgacgtt cccactatct tacaaggcgc
      241 taaattgttc agtctagatt tggccgcatt aaccgcaggt gctaaataca aaggtgattt
      301 cgaagaaaga ttcaaaggtg ttttgaagga aatcgaagaa tcaaagactc taattgtgtt
      361 attcattgat gaaattcaca tgttaatggg taatggtaag gacgacgctg ctaacatctt
      421 gaagccagct ttgtccagag gccaattgaa ggtcatcggt gccaccacca ataacgaata
      481 tagatctatt gtggaaaagg atggtgcctt tgaaagaaga ttccagaaaa ttgaagtcgc
      541 tgaaccaagt gtgagacaaa cagtggccat attgagaggt ctgcaaccaa agtatgaaat
      601 acatcatggt gtaaggattc tggatagcgc cttagtcacc gngnnnnacc ccctnttttt
      661 gtccccttta ncgagggtta atttcgagct tggcgtaatc atggtcatag ctgtttcctg
      721 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
      781 agccnggggt gcnaangagn gactacctca cntaattgcg tgcgctcctg cccctttcna
      841 ncnggaaacn gctggcngcn gctnataatn anc
//
