LOCUS       sequence-165066-         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For the Supernova, a series of vector systems for single-cell 
            labeling and gene function sudies in vivo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Mizuno H, Luo W, Tarusawa E, Saito YM, Sato T, Yoshimura Y, Itohara 
            S, Iwasato T
  TITLE     NMDAR-regulated dynamics of layer 4 neuronal dendrites during 
            thalamocortical reorganization in neonates.
  JOURNAL   Neuron. 2014 Apr 16;82(2):365-79. doi: 10.1016/j.neuron.2014.02.026.
            Epub 2014 Mar 27.
  PUBMED    24685175
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2014.02.026"; journalName: "Neuron"; date: 
            "2014-04-16- 16"; volume: "82"; issue: "2"; pages: "365-79"
COMMENT     Sequence Label: pK029.CAG-loxP-stop-loxP-RFP-ires-tTA-WPRE
            (Su...
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(51..84)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             127..822
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
ORIGIN
        1 gtctggatct gacatggtaa gtaagcttgg gctgcaggtc gagggaccta ataacttcgt
       61 atagcataca ttatacgaag ttatatgtcg acggtaccgc gggcccggga tccaccggtc
      121 gccaccatga gcgagctgat caaggagaac atgcacatga agctgtacat ggagggcacc
      181 gtgaacaacc accacttcaa gtgcacatcc gagggcgaag gcaagcccta cgagggcacc
      241 cagaccatga agatcaaggt ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg
      301 gctaccagct tcatgtacgg cagcaaagcc ttcatcaacc acacccaggg catccccgac
      361 ttctttaagc agtccttccc tgagggcttc acatgggaga gaatcaccac atacgaagac
      421 gggggcgtgc tgaccgctac ccaggacacc agcttccaga acggctgcat catctacaac
      481 gtcaagatca acggggtgaa cttcccatcc aacggccctg tgatgcagaa gaaaacacgc
      541 ggctgggagg ccaacaccga gatgctgtac cccgctgacg gcggcctgag aggccacagc
      601 cagatggccc tgaagctcgt gggcgggggc tacctgcact gctccttcaa gaccacatac
      661 agatccaaga aacccgctaa gaacctcaag atgcccggct tccacttcgt ggaccacaga
      721 ctggaaagaa tcaaggaggc cgacaaagag acctacgtcg agcagcacga gatggctgtg
      781 gccaagtact gcgacctccc tagcaaactg gggcacagat gatgctctag atcgagttaa
      841 ttaaggattc cgcccc
//
