LOCUS       sequence-165087-         190 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Staphylococcus aureus (SaCas9) conjugated with GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 190)
  AUTHORS   Ye L, Wang J, Tan Y, Beyer AI, Xie F, Muench MO, Kan YW
  TITLE     Genome editing using CRISPR-Cas9 to create the HPFH genotype in 
            HSPCs: An approach for treating sickle cell disease and 
            beta-thalassemia.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Sep 20;113(38):10661-5. doi: 
            10.1073/pnas.1612075113. Epub 2016 Sep 6.
  PUBMED    27601644
REFERENCE   2  (bases 1 to 190)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1612075113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-09-20- 20"; volume: "113"; issue: "38"; pages: "10661-5"
COMMENT     Sequence Label: pX601-GFP
FEATURES             Location/Qualifiers
     source          1..190
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        57..132
                     /label=Sa gRNA scaffold
                     /note="guide RNA scaffold for the Staphylococcus aureus 
                     CRISPR/Cas9 system"
ORIGIN
        1 ttggctttat atatcttgtg gaaaggacga aacaccggag accacggcag gtctcagttt
       61 tagtactctg gaaacagaat ctactaaaac aaggcaaaat gccgtgttta tctcgtcaac
      121 ttgttggcga gatttttgcg gccgcaggaa cccctagtga tggagttggc cactccctct
      181 ctgcgcgctc
//
