LOCUS       sequence-16511-e         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Cashikar AG, Schirmer EC, Hattendorf DA, Glover JR, Ramakrishnan MS,
            Ware DM, Lindquist SL
  TITLE     Defining a pathway of communication from the C-terminal peptide 
            binding domain to the N-terminal ATPase domain in a AAA protein.
  JOURNAL   Mol Cell 2002 Apr;9(4):751-60.
  PUBMED    11983167
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell"; 
            date: "2002-04"; volume: "9(4)"; pages: "751-60"
COMMENT     Sequence Label: pJC45BS104 F130W
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             17..39
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             52..81
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             94..105
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 aattccctct gaatattttg tttaacttta agaaggagat ataccatggg ccatcatcat
       61 catcatcatc atcatcatca cagcagcggc catatcgaag gtcgtggatc cgggatgaac
      121 gaccaaacgc aatttacaga aagggctcta acgattttga cgttggctca aaaattggct
      181 tcggatcatc aacatccaca attacaacct atacatattc tagctgcctt cattgaaacg
      241 ccagaagatg gatcagtccc ttacctacag aatctaattg agaagggccg ttacgactat
      301 gatcttttca agaaagtggt taatagaaat ctagtaagaa ttcctcaaca gcaacctgca
      361 cctgcggaga taactccaag ttatgctttg gggaaagtcc ttcaagacgc tgctaagatt
      421 caaaaacaac agaaggactc atttatagcg caagaccata tattgtttgc tctattcaat
      481 gattcgtcta ttcagcaaat atggaaggaa gctcaagtag atattgaggc catcaagcaa
      541 caagctcttg aacttcgtgg taacactaga attgactctc gtggcgctga tacgaacaca
      601 cctttggaat atttatcaaa gtacgccatt gatatgactg agcaggctcg tcaaggtaaa
      661 cttgaccctg tcatcggccg tgaagaagaa ataagaagca ctattagagt tttagcaaga
      721 agaattaagt ccaacccatg tttaattggt gagccaggta tcggtaagac cgctattatt
      781 gaaggtgttg ctcaaagaat cattgacgat gacgttccca ctatcttaca aggcgctaaa
      841 ttgttcagtc tagatttggc cgcattaacc gcaggtgcta a
//
