LOCUS       sequence-165110-         989 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For the Supernova, a series of vector systems for single-cell 
            labeling and gene function sudies in vivo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Mizuno H, Luo W, Tarusawa E, Saito YM, Sato T, Yoshimura Y, Itohara 
            S, Iwasato T
  TITLE     NMDAR-regulated dynamics of layer 4 neuronal dendrites during 
            thalamocortical reorganization in neonates.
  JOURNAL   Neuron. 2014 Apr 16;82(2):365-79. doi: 10.1016/j.neuron.2014.02.026.
            Epub 2014 Mar 27.
  PUBMED    24685175
REFERENCE   2  (bases 1 to 989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2014.02.026"; journalName: "Neuron"; date: 
            "2014-04-16- 16"; volume: "82"; issue: "2"; pages: "365-79"
COMMENT     Sequence Label: pK029.CAG-loxP-stop-loxP-RFP-ires-tTA-WPRE
            (Su...
FEATURES             Location/Qualifiers
     source          1..989
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(134..751,752..757,758..793,794..796,797..832,833..835,
                     836..871,872..880)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=tTA-Advanced
                     /note="In the Tet-Off(R) system, tTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(967..987)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca
       61 tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggtttcc
      121 tttgaaaaac acgatgtcta gactggacaa gagcaaagtc ataaactctg ctctggaatt
      181 actcaatgaa gtcggtatcg aaggcctgac gacaaggaaa ctcgctcaaa agctgggagt
      241 tgagcagcct accctgtact ggcacgtgaa gaacaagcgg gccctgctcg atgccctggc
      301 aatcgagatg ctggacaggc atcataccca cttctgcccc ctggaaggcg agtcatggca
      361 agactttctg cggaacaacg ccaagtcatt ccgctgtgct ctcctctcac atcgcgacgg
      421 ggctaaagtg catctcggca cccgcccaac agagaaacag tacgaaaccc tggaaaatca
      481 gctcgcgttc ctgtgtcagc aaggcttctc cctggagaac gcactgtacg ctctgtccgc
      541 cgtgggccac tttacactgg gctgcgtatt ggaggatcag gagcatcaag tagcaaaaga
      601 ggaaagagag acacctacca ccgattctat gcccccactt ctgagacaag caattgagct
      661 gttcgaccat cagggagccg aacctgcctt ccttttcggc ctggaactaa tcatatgtgg
      721 cctggagaaa cagctaaagt gcgaaagcgg cgggccggcc gacgcccttg acgattttga
      781 cttagacatg ctcccagccg atgcccttga cgactttgac cttgatatgc tgcctgctga
      841 cgctcttgac gattttgacc ttgacatgct ccccgggtaa ctaagtaagc ggccgcgaat
      901 tcgcccttcg gccgctcaac ctctggatta caaaatttgt gaaagattga ctggtattct
      961 taactatgtt gctcctttta cgctatgtg
//
