LOCUS       sequence-165114-         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Useful for tagging yeast proteins with C-terminal Venus tag; G418 
            selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Hailey DW, Davis TN, Muller EG
  TITLE     Fluorescence resonance energy transfer using color variants of green
            fluorescent protein.
  JOURNAL   Methods Enzymol. 2002;351:34-49.
  PUBMED    12073355
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2002"; volume: "351"; pages: "34-49"
COMMENT     Sequence Label: pBS7
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        54..72
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     54..71
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(160..188)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     783..803
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 tcgttagaac gcggctacaa ttaatacata accttatgta tcatacacat acgatttagg
       61 tgacactata gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatcccc
      121 gggttaatta acagtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa
      181 ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca
      241 acatacggaa aacttaccct taaattgatt tgcactactg gaaaactacc tgttccatgg
      301 ccaacacttg tcactacttt gggttatggt ctaatgtgct ttgctagata cccagatcat
      361 atgaaacggc atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaagaact
      421 atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt tgaaggtgat
      481 acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt
      541 ggacacaaat tggaatacaa ctataactca cacaatgtat acatcactgc agacaaacaa
      601 aagaatggaa tcaaagctaa cttcaaaatt agacacaaca ttgaagatgg aggtgttcaa
      661 ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac
      721 aaccattacc tgtcctatca atctgccctt tcgaaagatc ccaacgaaaa gagagaccac
      781 atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga tgaactatac
      841 aaatagggcg cgccacttct aaataagcga atttcttatg atttatgatt tttattatta
      901 aataagttat aaaaaa
//
